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This volume serves to aid researchers working in the recombinant
protein production field by describing a wide number of protocols
and examples. Chapters describe recombinant protein production in
different expression systems, prokaryotic and eukaryotic expression
systems, purification protocols, characterization of insoluble
proteins and a general overview of interesting applications of
insoluble proteins. Written in the format of the highly successful
Methods in Molecular Biology series, each chapter includes an
introduction to the topic, lists necessary materials and reagents,
includes tips on troubleshooting and known pitfalls, and
step-by-step, readily reproducible protocols. Authoritative and
cutting-edge, Insoluble Proteins: Methods and Protocols, Second
Edition aims to be a useful practical guide to researches to help
further their study in this field.
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