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As a high throughput method for analyzing gene function, cell-based
microarrays have proven to be of vital importance, allowing high
throughput analysis of over expression and knock down of proteins.
In Cell-Based Microarrays: Methods and Protocols, experts in the
field provide an up to date synopsis of cell-based microarrays and
meticulous coverage of all aspects of the array, including emerging
technology. Beginning with a detailed overview of the whole subject
area, the volume continues with protocols for over-expression
arrays and downstream functional assays, infectious disease
research, increasing transfection efficiencies, as well as the
development of cell-based array technology by use of microfluidic
image cytometry for the analysis of small diagnostic samples with
few cells. Written in the highly successful Methods in Molecular
Biology(TM) series format, chapters include introductions to their
respective topics, lists of the necessary materials and reagents,
step-by-step, readily reproducible laboratory protocols, and notes
on troubleshooting and avoiding known pitfalls. Comprehensive and
cutting-edge, Cell-Based Microarrays: Methods and Protocols serves
as a key resource for molecular biologists, geneticists,
immunologists, and chemists, and supplies scientists with access to
set up a technology that is truly high throughput for the
functional analysis of proteins.
As a high throughput method for analyzing gene function, cell-based
microarrays have proven to be of vital importance, allowing high
throughput analysis of over expression and knock down of proteins.
In Cell-Based Microarrays: Methods and Protocols, experts in the
field provide an up to date synopsis of cell-based microarrays and
meticulous coverage of all aspects of the array, including emerging
technology. Beginning with a detailed overview of the whole subject
area, the volume continues with protocols for over-expression
arrays and downstream functional assays, infectious disease
research, increasing transfection efficiencies, as well as the
development of cell-based array technology by use of microfluidic
image cytometry for the analysis of small diagnostic samples with
few cells. Written in the highly successful Methods in Molecular
Biology (TM) series format, chapters include introductions to their
respective topics, lists of the necessary materials and reagents,
step-by-step, readily reproducible laboratory protocols, and notes
on troubleshooting and avoiding known pitfalls. Comprehensive and
cutting-edge, Cell-Based Microarrays: Methods and Protocols serves
as a key resource for molecular biologists, geneticists,
immunologists, and chemists, and supplies scientists with access to
set up a technology that is truly high throughput for the
functional analysis of proteins.
This book is a review on the evolution of cell-based microarrays
and an update to the author's earlier book Methods in Molecular
Biology: Cell-Based Microarrays. Since their development in 2001,
cell-based microarrays have advanced significantly to include
expression arrays, short interfering RNA arrays and antibody
arrays. The surface used to coat the glass slides has also been
significantly improved to allow non-adherent cells to bind to the
arrays.
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