As a high throughput method for analyzing gene function, cell-based microarrays have proven to be of vital importance, allowing high throughput analysis of over expression and knock down of proteins. In Cell-Based Microarrays: Methods and Protocols, experts in the field provide an up to date synopsis of cell-based microarrays and meticulous coverage of all aspects of the array, including emerging technology. Beginning with a detailed overview of the whole subject area, the volume continues with protocols for over-expression arrays and downstream functional assays, infectious disease research, increasing transfection efficiencies, as well as the development of cell-based array technology by use of microfluidic image cytometry for the analysis of small diagnostic samples with few cells. Written in the highly successful Methods in Molecular Biology(TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Cell-Based Microarrays: Methods and Protocols serves as a key resource for molecular biologists, geneticists, immunologists, and chemists, and supplies scientists with access to set up a technology that is truly high throughput for the functional analysis of proteins.

This book is a review on the evolution of cell-based microarrays and an update to the author's earlier book Methods in Molecular Biology: Cell-Based Microarrays. Since their development in 2001, cell-based microarrays have advanced significantly to include expression arrays, short interfering RNA arrays and antibody arrays. The surface used to coat the glass slides has also been significantly improved to allow non-adherent cells to bind to the arrays.