This book constitutes the proceedings of the 13th International Conference on Simulation of Adaptive Behavior, SAB 2014, held in Castellon, Spain, in July 2014. The 32 papers presented in this volume were carefully reviewed and selected for inclusion in the proceedings. They cover the main areas in animat research, including the animat approach and methodology, perception and motor control, navigation and internal world models, learning and adaptation, evolution and collective and social behavior.