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This volume provides a wide range of protocols used in studying the
nuclear envelope, with special attention to the experimental
adjustments that may be required to successfully investigate this
complex organelle in cells from various organisms. The Nuclear
Envelope: Methods and Protocols is divided into five sections: Part
I - Nuclear Envelope Isolation; Part II - Nuclear Envelope Protein
Interactions, Localization, and Dynamics; Part III - Nuclear
Envelope Interactions with the Cytoskeleton; Part IV - Nuclear
Envelope-Chromatin Interactions; and Part V - Nucleo-Cytoplasmic
Transport. Many of the modifications discussed in this book have
only been circulated within laboratories that have conducted
research in this field for many years. Written in the highly
successful Methods in Molecular Biology series format, chapters
include introductions to their respective topics, lists of the
necessary materials and reagents, step-by-step, readily
reproducible laboratory protocols, and tips on troubleshooting and
avoiding known pitfalls. Cutting edge and thorough, The Nuclear
Envelope: Methods and Protocols is a timely resource for
researchers who have joined this dynamic and rapidly growing field.
"Nuclear envelope (NE) defects have been linked to cancer biology
since the mid-1800s, but it was not until the last few years that
we have begun to understand these historical links and to realize
that there are myriad ways that the NE impacts on tumorigenesis.
The NE is a complex double membrane system that encloses the genome
while providing structural support through the intermediate
filament lamin polymer and regulating protein/ mRNA trafficking and
signaling between the nucleus and cytoplasm via the nuclear pore
complexes (NPCs). These functions already provide some mechanisms
for NE influences on cancer biology but work in the past few years
has elucidated many others. Lamins and many recently identified NE
transmembrane proteins (NETs) have been now shown to function in
DNA repair, regulation of cell cycle and signaling, apoptosis, cell
migration in metastasis and nuclear architecture and morphology.
This volume presents a comprehensive overview of the wide range of
functions recently identified for NE proteins and their relevance
in cancer biology, providing molecular mechanisms and evidence of
their value as prognostic and diagnostic markers and suggesting new
avenues for the treatment of cancer. Indeed some of these recent
links are already yielding promising therapies, such as the current
clinical trial of selective inhibitors of the nuclear export factor
exportin in certain types of leukemia, melanoma and kidney cancer."
This volume provides a wide range of protocols used in studying the
nuclear envelope, with special attention to the experimental
adjustments that may be required to successfully investigate this
complex organelle in cells from various organisms. The Nuclear
Envelope: Methods and Protocols is divided into five sections: Part
I - Nuclear Envelope Isolation; Part II - Nuclear Envelope Protein
Interactions, Localization, and Dynamics; Part III - Nuclear
Envelope Interactions with the Cytoskeleton; Part IV - Nuclear
Envelope-Chromatin Interactions; and Part V - Nucleo-Cytoplasmic
Transport. Many of the modifications discussed in this book have
only been circulated within laboratories that have conducted
research in this field for many years. Written in the highly
successful Methods in Molecular Biology series format, chapters
include introductions to their respective topics, lists of the
necessary materials and reagents, step-by-step, readily
reproducible laboratory protocols, and tips on troubleshooting and
avoiding known pitfalls. Cutting edge and thorough, The Nuclear
Envelope: Methods and Protocols is a timely resource for
researchers who have joined this dynamic and rapidly growing field.
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