The field of non-viral vector research has rapidly progressed since the publication of the first edition. This new edition is expanded to two separate volumes that contain in-depth discussions of different non-viral approaches, including cationic liposomes and polymers, naked DNA and various physical methods of delivery, as well as a comprehensive coverage of the molecular biological designs of the plasmid DNA for reduced toxicity, prolonged expression and tissue or disease specific genes. New developments such as the toxicity of the non-viral vectors and recent advances in nucleic acid therapeutics are fully covered in these volumes.

Acupuncture: a scientific appraisal takes an evaluative approach, summarising the evidence for acupuncture in an impartial way. An international team of specialist contributors brings a wide range of expertise to the book, drawing together different facets of research and clinical practice to provide a cohesive look at acupuncture today. The increasing integration of acupuncture into health care services in the west, requires stringent criteria of efficacy, safety and cost in order to provide optimal benefit for patients, at minimal risk. All professionals involved in the delivery of healthcare will find this new book of great interest. The conclusions reached should provide a focus for future debate. Fully referenced throughout, Acupuncture: a scientific appraisal is an easily accessible, balanced source of information for all practitioners of acupuncture. Healthcare providers, complementary therapists and members of the primary healthcare team will also find this an invaluable purchase. * examines acupuncture's possible mechanisms of action * contains chapters on both Eastern and Western approaches * reviews the evidence for the effectiveness of acupuncture

Even during the artist's lifetime, contemporary art lovers considered Rembrandt van Rijn (1606-1669) to be an exceptional artist. In this revelatory sequel to the acclaimed Rembrandt: The Painter at Work, renowned Rembrandt authority Ernst van de Wetering investigates the painter's considerations that determined the striking changes in his development from an early age onwards. This gorgeously illustrated book explores how Rembrandt achieved mastery by systematic exploration of the 'foundations of the art of painting'. According to written sources from the seventeenth century, which were largely misinterpreted until now, these 'foundations' were considered essential at that time. From his first endeavours in painting, Rembrandt embarked on a journey past these foundations, thus becoming the 'pittore famoso', whom Count Cosimo the Medici visited at the end of his life. Rembrandt never stopped searching for solutions to the pictorial problems that confronted him; this led over time to radical changes that cannot simply be attributed to stylistic evolution or natural development. In a quest as rigorous and novel as the artist's, Van de Wetering reveals how Rembrandt became the revolutionary painter that would continue to fascinate the art world. This ground breaking exploration reconstructs Rembrandt's theories and methods, shedding new light both on the artist's exceptional accomplishments and on the theory and practice of painting in the Dutch Golden Age. Everyone who is interested in the art of painting should read this phenomenal book, because it was written with incredible knowledge and experience on the subject. It shows in a clear and simple way how Rembrandt worked and the things he had to take into account. At the same time it offers a fantastic sample of Rembrandt's life's work, thanks to the well-chosen selection of illustrations. David Rijser, NRC Handelsblad

Even during the artist's lifetime, contemporary art lovers considered Rembrandt van Rijn (1606-1669) to be an exceptional artist. In this revelatory sequel to the acclaimed Rembrandt: The Painter at Work, renowned Rembrandt authority Ernst van de Wetering investigates the painter's considerations that determined the striking changes in his development from an early age onwards. This gorgeously illustrated book explores how Rembrandt achieved mastery by systematic exploration of the 'foundations of the art of painting'. According to written sources from the seventeenth century, which were largely misinterpreted until now, these 'foundations' were considered essential at that time. From his first endeavours in painting, Rembrandt embarked on a journey past these foundations, thus becoming the 'pittore famoso', whom Count Cosimo the Medici visited at the end of his life. Rembrandt never stopped searching for solutions to the pictorial problems that confronted him; this led over time to radical changes that cannot simply be attributed to stylistic evolution or natural development. In a quest as rigorous and novel as the artist's, Van de Wetering reveals how Rembrandt became the revolutionary painter that would continue to fascinate the art world. This ground breaking exploration reconstructs Rembrandt's theories and methods, shedding new light both on the artist's exceptional accomplishments and on the theory and practice of painting in the Dutch Golden Age.