This volume provides a comprehensive look at the current fabrication methods, materials, cell patterning techniques, and practical application of live-cell microarrays. The chapters in this book are categorized into six parts: introduction, biointerface engineering and live-cell microarrays, printing techniques for live-cell microarrays, live-cell microarray application, microfluidic live-cell arrays, and advanced materials suitable for live-cell microarrays. Some of the topics covered are artificial micro-niche array with spatially structured biochemical cues; inkjet printing for biomedical application; fabrication of multi-electrode arrays for neurobiology applications; rapid prototyping of thermoplastic microfluidic devices; and oxygen-generating photocrosslinkable hydrogel. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Cell-Based Microarrays: Methods and Protocols is a valuable resource for anyone interested in expanding their knowledge and research of cell-based microarrays.

This book provides an outline of theoretical concepts and their experimental verification in studies of self-organization phenomena in chemical systems, as they emerged in the mid-20th century and have evolved since. Presenting essays on selected topics, it was prepared by authors who have made profound contributions to the field. Traditionally, physical chemistry has been concerned with interactions between atoms and molecules that produce a variety of equilibrium structures - or the 'dead' order - in a stationary state. But biological cells exhibit a different 'living' kind of order, prompting E. Schroedinger to pose his famous question "What is life?" in 1943. Through an unprecedented theoretical and experimental development, it was later revealed that biological self-organization phenomena are in complete agreement with the laws of physics, once they are applied to a special class of thermodynamically open systems and non-equilibrium states. This knowledge has in turn led to the design and synthesis of simple inorganic systems capable of self-organization effects. These artificial 'living organisms' are able to operate on macroscopic to microscopic scales, even down to single-molecule machines. In the future, such research could provide a basis for a technological breakthrough, comparable in its impact with the invention of lasers and semiconductors. Its results can be used to control natural chemical processes, and to design artificial complex chemical processes with various functionalities. The book offers an extensive discussion of the history of research on complex chemical systems and its future prospects.

Technologies and Practices for Constructing Knowledge in Online Environments: Advancements in Learning discusses how aspects of technology can facilitate and provide advancements in e-collaborative knowledge construction. This reference collection gives an impression about scenarios of e-collaborative knowledge construction and the technology applied in these scenarios while focusing on technologies that enable collaborative knowledge construction processes and how they can be framed to support e-collaborative knowledge construction.

DNA Vaccines: An Introduction; M.R. Hilleman. Architecture of a DNA vaccine; G. Pavlakis. DNA vaccine delivery; S. Kaufmann. Adjuvanticity of DNA vaccines; A. Krieg. Immune responses to DNA vaccines: Antigen presentation; R. Steinman. Immune responses to DNA vaccines: Antigen processing; J. Yewdell. Immune responses to DNA vaccines: Induction of B cells; G. Kelsoe. Immune responses to DNA vaccines: Induction of CD4+ T cells; E. Shevach. Immune responses to DNA vaccines: Induction of CD8+ T cells; L. Whitton. Immune responses to DNA vaccines: Cytokines as immune mediators as part of the immune response and their potential as genetic adjuvants to DNA vaccines; H. Ertl. Immune responses to DNA vaccines: Chemokines as immune mediators as part of the immune response and their potential as genetic adjuvants to DNA vaccines; P. Murphy. DNA Vaccines to infectious agents: RNA viruses; J. Ulmer. DNA Vaccines to infectious agents: HIV/SIV; B. Wahren. DNA Vaccines to infectious agents: DNA viruses; B. Rouse. DNA Vaccines to infectious agents: Tumor-associated viruses (excluding HBV); R. Kennedy. DNA Vaccines to infectious agents: Bacteria; D. Lowrie. DNA Vaccines to infectious agents: Parasites; S. Hoffman. Use of DNA vaccines for neonatal/early childhood immunization; C.-A. Siegrist. The potential of DNA vaccines for developing countries; H. Wilde. DNA vaccines and their potential to counterbalance biological warfare/bioterrorism; A. Schmaljohn. DNA vaccines to cancer associated/specific antigens; DNA vaccines to autoimmune diseases; H. Wigzell. DNA vaccines to allergic diseases; Yan Chuah, P. Holt. DNA vaccines for gene therapy; K. High. Safety concerns for DNA; D. Klinman. DNA vaccines: Summary.

One of the greatest scientific challenges of the 21st century is how to master, organize and extract useful knowledge from the overwhelming flow of information made available by today 's data acquisition systems and computing resources. Visualization is the premium means of taking up this challenge. This book is based on selected lectures given by leading experts in scientific visualization during a workshop held at Schloss Dagstuhl, Germany. Topics include user issues in visualization, large data visualization, unstructured mesh processing for visualization, volumetric visualization, flow visualization, medical visualization and visualization systems. The book contains more than 350 color illustrations.

Like other industrial nations, Japan is experiencing its own forms of, and problems with, internationalization and multiculturalism. This volume focuses on several aspects of this process and examines the immigrant minorities as well as their Japanese recipient communities. Multiculturalism is considered broadly, and includes topics often neglected in other works, such as: religious pluralism, domestic and international tourism, political regionalism and decentralization, sports, business styles in the post-Bubble era, and the education of immigrant minorities.

Like other industrial nations, Japan is experiencing its own forms of, and problems with, internationalization and multiculturalism. This volume focuses on several aspects of this process and examines the immigrant minorities as well as their Japanese recipient communities. Multiculturalism is considered broadly, and includes topics often neglected in other works, such as: Religious pluralism, domestic and international tourism, political regionalism and decentralization, sports, business styles in the post-Bubble era, archaeological interpretation of Japanese-Korean origins, blacks and stateless people in Japan.

Of late, historians have been realising that South Asia and Europe have more in common than a particular strand in the historiography on "the rise of the West" would have us believe. In both world regions a plurality of languages, religions, and types of belonging by birth was in premodern times matched by a plurality of legal systems and practices. This volume describes case-by-case the points where law and social diversity intersected.

The Routledge International Handbook of Gender Beliefs, Stereotype Threat, and Teacher Expectations presents, for the first time, the work of leading researchers exploring the synergies and interrelationships between these fields, and provides a catalytic platform for advancing theory, practice, policy and research from an integrated perspective. An understanding of how gender beliefs, stereotype threat and teacher expectations interrelate is vital to creating safe, equitable and encouraging learning spaces. The collection summarises how gender beliefs, stereotype threat and teacher expectations act in association to influence gendered student achievement, engagement and self-beliefs, and suggests ways toward rectifying their negative effects. The chapters are organised into four sections: Gender Beliefs, Identity, Stereotypes, and Student Futures, Stereotype Threat, Teacher Expectations, Synergies and Solutions, By examining synergies and solutions shared between the three fields this book creates more meaningful, consistent, and permanent approaches to achieving gender identity safety, gendered scholastic equity, wellbeing and positive futures for students. This comprehensive publication brings together cutting-edge research at the intersection of gender beliefs, stereotype threat and teacher expectations. It is an essential reference for researchers and postgraduate students in education and gender studies as well as educational, social and developmental psychology.

Now in 8 volumes, the completely revised and expanded second edition of this much-cited handbook collates the knowledge available on heterogeneous catalysis, providing easy-to-find yet comprehensive information. The new edition contains some 80% more material and takes into account the latest developments in the field, making it still the most up-to-date compendium in heterogeneous catalysis. More than 300 leading experts -- a veritable "Who's Who" in catalysis -- contributed to this unrivalled masterpiece, covering all aspects of the subject, from the physico-chemical foundations to large-scale industrial applications.
With its straightforward presentation, this is an essential and indispensable tool for every scientist working in this area.

This study mirrors the perceptions and values that shape the discussion of such terms as harmonization, recognition, convergence and subsidiarity in the educational sphere. It provides insights into surprising similarities and important differences in the approaches of different Member States regarding the interpretation and implementation of EU education and training policies. It summarizes the results of a European research project conducted within the EU-funded network PRESTiGE.

This book centers on gene therapy and gene transfer approaches to prevent or treat chronic virus infections. The main focus is on the Big Three: human immunodeficiency virus (HIV-1), hepatitis B virus (HBV) and hepatitis C virus (HCV). Ample anti-HIV drugs are currently available in the clinic and the development of an effective combination therapy has dramatically improved the lifespan and quality of life of infected individuals. A similar trend can already be recognized for HBV and HCV: the development of multiple (directly acting) antiviral drugs and plans to control or even cure the infection. However, approaches that help prevent infection, or which provide long-lasting treatment (such as a cure) remain important goals. Immunization through gene transfer vehicles encoding immunogenic viral proteins shows promise in preventing infections with complex, highly variable, viruses such as HIV-1 or HCV. Gene therapy applications for virus infections have been discussed since the early 1990's. Whereas a true cure seems difficult to achieve for HIV-1 due to its intrinsic property to deposit its genome into that of the host, such attempts may be within reach for HCV where spontaneous viral clearance occurs in a small percentage of the infected individuals. The prospect of original gene therapy approaches may provide alternative ways to reach the same endpoint by, for example, silencing of CCR5 expression post-transcriptionally. Many alternative antiviral strategies have been developed based on a variety of novel molecular methods: e.g. ribozymes. Some studies have progressed towards pre-clinical animal models and a few antiviral gene therapies have progressed towards clinical trials. This book provides an overview of this rapidly progressing field, while focusing on the interface of gene therapy and immunology/vaccinology.

This second review volume is a follow-up to the book "Engineering of Chemical Complexity" that appeared in 2013. Co-edited by the Nobel laureate Gerhard Ertl, this book provides a broad perspective over the current research aimed at understanding, the design and control of complex chemical systems of various origins, on the scales ranging from single molecules and nano-phenomena to macroscopic chemical reactors. Self-organization behavior and emergence of coherent collective dynamics in reaction-diffusion systems, in active soft matter and biochemical networks are discussed. Special attention is paid to applications in cell biology, to molecular motors and microfluidics effects. The reviews, prepared by leading international experts from the EU, USA, Russia and Japan, together yield a fascinating picture of a rapidly developing research discipline that brings chemical engineering to new frontiers.

This review volume, co-edited by Nobel laureate G Ertl, provides a broad overview on current studies in the understanding of design and control of complex chemical systems of various origins, on scales ranging from single molecules and nano-phenomena to macroscopic chemical reactors. Self-organizational behavior and the emergence of coherent collective dynamics in reaction diffusion systems, reactive soft matter and chemical networks are covered. Special attention is paid to the applications in molecular cell biology and to the problems of biological evolution, synthetic biology and design of artificial living cells. Starting with a detailed introduction on the history of research on complex chemical systems, its current state of the art and perspectives, the book comprises 19 chapters that survey the current progress in particular research fields. The reviews, prepared by leading international experts, yield together a fascinating picture of a rapidly developing research discipline that brings chemical engineering to new frontiers.

Modern mundane life is brimming with a variety of data-driven technologies that are supposed to augment the practices they are involved in. As humans bring these technologies into their lives in a process of domestication, they tame them and are simultaneously influenced by their presence. In combining domestication research and an empirical analysis of current, digital, and interconnected media, this issue examines the process of taming with an emphasis on practices. The contributions in this issue explore the use of digitally connected media such as vacuum robots, smart speakers, drones, and kitchen appliances with reference to the domestication paradigm from interdisciplinary perspectives including media studies, sociology, anthropology, and human-computer interaction.

Policies to promote high participation in Higher Educations (HE) systems aim to deliver social justice and economic development through widening participation of under-represented groups. Degrees of Success provides a critical test of this through examination of participation and success of learners progressing to HE with a vocational background. Employing an original conceptual framework that combines the ideas of Basil Bernstein and Pierre Bourdieu the authors analyse the various transitional frictions experienced by learners with VET backgrounds on their journeys into and through the HE system. The findings indicate that including students with vocational qualifications does lead to widening participation but that their modes of participation may not provide fair access and outcomes. In part this is due to the epistemic incompatibilities between higher and vocational education which remain unresolved despite constant VET qualification reform. This book, therefore, extends the debate about widening participation beyond metaphors of barriers to access to consider the epistemic and pedagogical challenges of increasing student heterogeneity in high participation HE systems. The analysis and policy suggestions therefore have relevance for all seeking to support students' HE learning journeys, and policy makers concerned with how best to utilise HE systems as means of furthering social mobility and justice.

Improving Learning by Widening Participation in Higher Education presents a strong and coherent rationale for improving learning for diverse students from a range of socio-economic, ethnic/racial and gender backgrounds within higher education, and for adults across the life course.

Edited by Miriam David, the Associate Director of the ESRC s highly successful Teaching and Learning Research Programme, with contributions from the seven projects on Widening Participation in Higher Education (viz Gill Crozier and Diane Reay; Chris Hockings; Alison Fuller and Sue Heath; Anna Vignoles; Geoff Hayward and Hubert Ertl; Julian Williams and Pauline Davis; Gareth Parry and Ann-Marie Bathmaker), this book provides clear and comprehensive research evidence on the policies, processes, pedagogies and practices of widening or increasing participation in higher education. This evidence is situated within the contexts of changing individual and institutional circumstances across the life course, and wider international transformations of higher education in relation to the global knowledge economy.

Improving Learning by Widening Participation in Higher Education also considers:

• the changing UK policy contexts of post-compulsory education;
• how socio-economically disadvantaged students raced and gendered fare through schools and into post-compulsory education;
• the kinds of academic and vocational courses, including Maths, undertaken;
• the changing forms of institutional and pedagogic practices within higher education;
• how adults view the role of higher education in their lives.

This book, based upon both qualitative studies and quantitative datasets, offers a rare insight into the overall implications for current and future policy and will provide a springboard for further research and debate. It will appeal both to policy-makers and practitioners, as well as students within higher education.

This Element addresses three questions about Kant's guarantee thesis by examining the 'first addendum' of his Philosophical Sketch: how the guarantor powers interrelate, how there can be a guarantee without undermining freedom and why there is a guarantee in the first place. Kant's conception of an interplay of human and divine rational agency encompassing nature is crucial: on moral grounds, we are warranted to believe the 'world author' knew that if he were to bring about the world, the 'supreme' good would come about too. Perpetual peace is the condition that enables the supreme good to be realized in history.