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In addition to supplementing existing analysis techniques in
serious crime cases, trace DNA can allow investigation of volume
crime cases such as burglary or vehicle theft, where previously DNA
evidence was not considered usable. However, despite the widespread
use of trace DNA, at present there are very few specific validated
methods. This has lead to controversy in the use of trace DNA, and
particularly the low copy number amplification technique. It has
been established that the use of existing methodology (developed
for high-copy number samples) leads to significant levels of
artefacts with trace DNA, including allele drop-out and drop-in,
stutter, and allelic/locus imbalance. To minimise these, there are
numerous modifications that can be made to existed methods to
increase the success of trace DNA analysis. This book presents
advances in the field of DNA research as an aid in Forensic studies
and Genetics.
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