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This volume details methods of identifying synthetic lethal,
genetic interactions by various approaches in different model
systems including human cancer cells. Chapters guide readers
through genetic interactions in model organisms, RNA interference,
CRISPR/Cas9 based genome editing technologies, drug-gene
interactions, mapping chemical genetic interactions, synergistic
drug-gene relations, single cell sequencing, gene expression
profiling, and novel genetic interactions. Written in the format of
the highly successful Methods in Molecular Biology series, each
chapter includes an introduction to the topic, lists necessary
materials and reagents, includes tips on troubleshooting and known
pitfalls, and step-by-step, readily reproducible protocols.
Authoritative and cutting-edge, Genetic Interaction Mapping aims to
be a useful practical guide to researches to help further their
study in this field.
This volume details methods of identifying synthetic lethal,
genetic interactions by various approaches in different model
systems including human cancer cells. Chapters guide readers
through genetic interactions in model organisms, RNA interference,
CRISPR/Cas9 based genome editing technologies, drug-gene
interactions, mapping chemical genetic interactions, synergistic
drug-gene relations, single cell sequencing, gene expression
profiling, and novel genetic interactions. Written in the format of
the highly successful Methods in Molecular Biology series, each
chapter includes an introduction to the topic, lists necessary
materials and reagents, includes tips on troubleshooting and known
pitfalls, and step-by-step, readily reproducible protocols.
Authoritative and cutting-edge, Genetic Interaction Mapping aims to
be a useful practical guide to researches to help further their
study in this field.
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