A readily reproducible collection of established and emerging techniques for studying the interaction between proteins and ligands, including biochemical/bulk techniques, structure analysis, spectroscopy, single-molecule studies, and theoretical/computational tools. Among the highlights are surface plasmon resonance (SPR) and reflectometric biosensor approaches, high-throughput screening with confocal optics microscopy, single molecule fluorescence and fluorescence correlation spectroscopy (FCS), atomic force microscopy (AFM), crystallography of reaction intermediates, and time-resolved x-ray crystallography. The protocols follow the successful Methods in Molecular Biologya"[ series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.

Physics and the life sciences have established new connections within the past few decades, resulting in biological physics as an established subfield with strong groups working in many physics departments. These interactions between physics and biology form a two-way street with physics providing new tools and concepts for understanding life, while biological systems can yield new insights into the physics of complex systems. To address the challenges of this interdisciplinary area, The Physics of Proteins: An Introduction to Biological Physics and Molecular Biophysics is divided into three interconnected sections. In Parts I and II, early chapters introduce the terminology and describe the main biological systems that physicists will encounter. Similarities between biomolecules, glasses, and solids are stressed with an emphasis on the fundamental concepts of living systems. The central section (Parts III and IV) delves into the dynamics of complex systems. A main theme is the realization that biological systems, in particular proteins, do not exist in unique conformations but can assume a very large number of slightly different structures. This complexity is captured in the concept of a free energy landscape and leads to the conclusion that fluctuations are crucial for the functioning of biological systems. The final chapter of this section challenges the reader to apply these concepts to a problem that appears in the current literature. An extensive series of appendices (Part V) provide descriptions of the key physical tools and analytical methods that have proven powerful in the study of the physics of proteins. The appendices are designed to be consulted throughout the section on protein dynamics without breaking the deductive flow of the logic in the central section of the book.

The moral theology of Hans G. Ulrich is presented here in English for the first time. These collected essays represent the culmination of a lifetime of reflection on Christian living from this German theologian in conversation with Luther, Bonhoeffer, and contemporary philosophers and theologians. Ulrich's ethics affirm the lively presence of the living work of God in orienting the daily life of Christians. This presence enables members of the Church to live as creatures trusting in God's promises, bearing witness in political and economic spheres, and trusting in life as a gift in response to bioethical issues. Ulrich's fresh take on living out of the promise of God yields further guidance on issues in international relations, economics, parenting, disability, and more.

The two unique benefits of Concurrent and Comparative Discrete Event Simulation are: speed, which is usually 1000 to 10 000 times faster than conventional discrete event simulation; and methodology, which permits the concurrent/comparative simulation of many thousands of experiments. One idea is that a one-for-many experiment, called the reference, is simulated in its entirety, while all others are simulated only where they differ from the reference. A second idea extends the first one; many one-for-many experiments will be significantly more efficient than only one experiment. These two ideas result in tremendous efficiencies, permitting the concurrent simulation of tens of thousands of experiments. The material in the book covers a vast application area in the scientific and business world. For example, in the design experimentation of nuclear power plant operations, many scenarios can be simulated to derive desirable designs or safe operating procedures. Concurrent fault simulation is already a mature technique in the computer aided design of digital systems. Concurrent/Comparative Simulation (CCS) of several instruction sets for a computer can help a designer in making performance tradeoffs. One of the most powerful future applications for CCS/MDCCS (Concurrent and Comparative Simulation/Multi-Domain Concurrent and Comparative Simulation) will be in the testing and debugging of computer programs.

Concurrent simulation is over twenty years old. During that pe riod it has been widely adopted for the simulation of faults in digital circuits, for which it provides a combination of extreme efficiency and generality . Yet, it is remarkable that no book published so far presents a correct and sufficiently detailed treatment of concurrent simulation. A first reason to welcome into print the effort of the authors is, therefore, that it provides a much needed account of an important topic in design automation. This book is, however, unique for sev eral other reasons. It is safe to state that no individual has contrib uted more than Ernst Ulrich to the development of digital logic simulation. For concurrent simulation, one may say that Ernst has contributed more than the rest of the world. We would find such a claim difficult to dispute. The unique experience of the authors con fers a special character to this book: It is authoritative, inspired, and focused on what is conceptually important. Another unique aspect of this book, perhaps the one that will be the most surprising for many readers, is that it is strongly projected towards the future. Concurrent simulation is presented as a general experimentation methodology and new intriguing applications are analyzed. The discussion of multi-domain concurrent simulation-- recent work of Karen Panetta Lentz and Ernst Ulrich---is fascinat ing."

Physics and the life sciences have established new connections within the past few decades, resulting in biological physics as an established subfield with strong groups working in many physics departments. These interactions between physics and biology form a two-way street with physics providing new tools and concepts for understanding life, while biological systems can yield new insights into the physics of complex systems. To address the challenges of this interdisciplinary area, The Physics of Proteins: An Introduction to Biological Physics and Molecular Biophysics is divided into three interconnected sections. In Parts I and II, early chapters introduce the terminology and describe the main biological systems that physicists will encounter. Similarities between biomolecules, glasses, and solids are stressed with an emphasis on the fundamental concepts of living systems. The central section (Parts III and IV) delves into the dynamics of complex systems. A main theme is the realization that biological systems, in particular proteins, do not exist in unique conformations but can assume a very large number of slightly different structures. This complexity is captured in the concept of a free energy landscape and leads to the conclusion that fluctuations are crucial for the functioning of biological systems. The final chapter of this section challenges the reader to apply these concepts to a problem that appears in the current literature. An extensive series of appendices (Part V) provide descriptions of the key physical tools and analytical methods that have proven powerful in the study of the physics of proteins. The appendices are designed to be consulted throughout the section on protein dynamics without breaking the deductive flow of the logic in the central section of the book.

A readily reproducible collection of established and emerging techniques for studying the interaction between proteins and ligands, including biochemical/bulk techniques, structure analysis, spectroscopy, single-molecule studies, and theoretical/computational tools. Among the highlights are surface plasmon resonance (SPR) and reflectometric biosensor approaches, high-throughput screening with confocal optics microscopy, single molecule fluorescence and fluorescence correlation spectroscopy (FCS), atomic force microscopy (AFM), crystallography of reaction intermediates, and time-resolved x-ray crystallography. The protocols follow the successful Methods in Molecular Biology (TM) series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.

The moral theology of Hans G. Ulrich is presented here in English for the first time. These collected essays represent the culmination of a lifetime of reflection on Christian living from this German theologian in conversation with Luther, Bonhoeffer, and contemporary philosophers and theologians. Ulrich's ethics affirm the lively presence of the living work of God in orienting the daily life of Christians. This presence enables members of the Church to live as creatures trusting in God's promises, bearing witness in political and economic spheres, and trusting in life as a gift in response to bioethical issues. Ulrich's fresh take on living out of the promise of God yields further guidance on issues in international relations, economics, parenting, disability, and more.

Die Notwendigkeit wissenschaftlicher Erkenntnis der physikalischen und chemischen Eigenschaften der Faserstoffe als Grundlage fur ihre zweck massige Verarbeitung und Verwendung ist immer mehr erkannt worden. Aus diesem Grunde hat auch die wissenschaftliche Wollforschung in letzter Zeit einen erheblichen Aufschwung genommen, begunstigt noch durch die all gem-eine Entwicklung der Faserphysik, Chemie und Kolloidehernie der Ei weissstoffe, sowohl in theoretischer als auch in methodischer Hinsicht. Im vorliegenden Band wird erstmalig eine umfassende Darstellung des gegen wartigen Standes der Strukturerforschung des Wollhaares und seines phy sikalischen und chemischen Verhaltens gegeben, wobei die gesamte neuere und altere Literatur nahezu vollstandig berucksichtigt ist. Fur den Fach mann besonders wertvoll ist auch die eingehende und kritische Zusammen stellung der qualitativen und quantitativen Methoden zur Untersuchung der Wolle, allein und in Fasergemischen. Weiterhin werden die chemischen Verfahren der Wollverarbeitung von praktischen Gesichtspunkten aus be handelt, und in einem 2. Teil folgt eine eingehende, von zahlreichen Abbil dungen begleitete Beschreibung der maschinellen Hilfsmittel fur die mecha nische und chemische Ausrustung von Wollwaren. Dem Herausgeber der bis jetzt erschienenen Bande dieses Handbuches, Herrn Prof. Dr. R. O. Herzog, der inzwischen verstorben ist, verdanken wir auch die Entstehung des vorliegenden Bandes, auf dessen Zielsetzung und Ausgestaltung er noch bestimmenden Einfluss hatte. Den Verfassern der einzelnen Teile dieses Bandes sei an dieser Stelle der verbindlichste Dank ausgesprochen."

Die „Geburtsstunde" der Fotografie im Jahr 1839 hat die internationale Bildwelt fasziniert und erschüttert zugleich. Nie zuvor war es möglich gewesen, Abbilder der Wirklichkeit so schnell und präzise zu erschaffen – und dies ganz ohne Pinsel oder Stift. Der Ausstellungskatalog erzählt die wechselvolle Geschichte von Malerei und Fotografie im 19. Jahrhundert, die geprägt ist von Konkurrenzangst, Experimentierfreude und Künstlerstolz. Er schlägt einen Bogen von der Medienrevolution 1839 bis in die Zeit um 1900, da die Fotografie erstmals auch als Kunstform breitere Anerkennung fand. Porträts und Aktdarstellungen, Orientbilder und Wolkenstudien, Architekturgemälde und Gedankenfotos setzen bedeutende Positionen von Malerei und Fotografie in Dialog. Rund 200 Werke zeigen die verschiedenen Potenziale beider Bildmedien, von der Inszenierung des Unwirklichen im Reich der Fantasie bis zur Gewinnung neuer Erkenntnisse im Dienst der Naturwissenschaft. Künstler/-innen: Johann Wilhelm Schirmer, Anselm Feuerbach, Hans Makart, James Tissot, Camille Pissarro, Lovis Corinth Fotografische Pionier/-innen: Charles Nègre, Francis Frith, Julia Margaret Cameron, Adolphe Braun, Josef Eder, Heinrich Kühn, Edward Steichen Ausstellung: Staatliche Kunsthalle Karlsruhe, 2019