The first section of this volume corresponds to courses on the cytoskeleton, its various structures and its dynamics, especially during the cell cycle. The reductionist approach is favoured in this field and considerable effort is spent on finding out how these structures are built up from their component molecules, how they grow or decrease in size, how they interact with each other and with other cell components. The second section describes the endo membrane system of a eukaryotic cell and the regulated protein traffic that flows through it. Part III deals with the onset of higher levels of organization. Topics covered include the development of the central nervous system, the role of time in biology and theoretical models to describe biochemical and cellular oscillations. The volume concludes with a reflection on physics and biology and the author shares some of his thoughts on the different ways in which physicists and biologists tackle problems in their respective fields.