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This manuscript discusses the potentials of the approaches as
mentioned below to monitor the AIVs in WMW. Molecular diagnostic
platforms enable for accurate detection of the AIVs in the feces of
infected birds. Similar technologies can be used to determine the
bird species through DNA barcoding, enabling non-invasive research
on the epidemiology of the disease. Wild migratory waterfowl (WMW)
play significant role in the transmission of avian influenza
viruses (AIVs) on large distances. Understanding bird migrations
may therefore significantly contribute towards understanding of the
disease epidemiology, however most conventional approaches to trace
WMW migrations are based on capturing, tagging (mostly ringing or
GPS devices) and their re-capturing to link the departure and
arrival places. Stable isotope ratios in metabolically inert
tissues (feathers, beaks, claws) reflect the ratios present at the
point of intake (drinking or feeding), thus enabling for tracing
bird origins at stopover places. Molecular diagnostic platforms
such as the polymerase chain reaction (PCR) enable for accurate
detection of the AIVs in the feces of infected birds. Similar
technologies (genetic sequencing) can be used to determine the bird
species through DNA barcoding. Simple and easy collection of
feather and fecal samples at the stopover places may generate a
full information package on which species of WMW carries the AIVs
(PCR+DNA barcoding on the feces), as well as the origin of these
species (SI+DNA barcoding on the feathers). Therefore, such
approaches enable for research on the epidemiology and the ecology
of the AIVs in WMW using a non-invasive platform, which does not
require capturing of WMW. This manuscript discusses the potentials
of these approaches to monitor the AIVs in WMW. p>
This manuscript discusses the potentials of the approaches as
mentioned below to monitor the AIVs in WMW. Molecular diagnostic
platforms enable for accurate detection of the AIVs in the feces of
infected birds. Similar technologies can be used to determine the
bird species through DNA barcoding, enabling non-invasive research
on the epidemiology of the disease. Wild migratory waterfowl (WMW)
play significant role in the transmission of avian influenza
viruses (AIVs) on large distances. Understanding bird migrations
may therefore significantly contribute towards understanding of the
disease epidemiology, however most conventional approaches to trace
WMW migrations are based on capturing, tagging (mostly ringing or
GPS devices) and their re-capturing to link the departure and
arrival places. Stable isotope ratios in metabolically inert
tissues (feathers, beaks, claws) reflect the ratios present at the
point of intake (drinking or feeding), thus enabling for tracing
bird origins at stopover places. Molecular diagnostic platforms
such as the polymerase chain reaction (PCR) enable for accurate
detection of the AIVs in the feces of infected birds. Similar
technologies (genetic sequencing) can be used to determine the bird
species through DNA barcoding. Simple and easy collection of
feather and fecal samples at the stopover places may generate a
full information package on which species of WMW carries the AIVs
(PCR+DNA barcoding on the feces), as well as the origin of these
species (SI+DNA barcoding on the feathers). Therefore, such
approaches enable for research on the epidemiology and the ecology
of the AIVs in WMW using a non-invasive platform, which does not
require capturing of WMW. This manuscript discusses the potentials
of these approaches to monitor the AIVs in WMW. p>
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