This second edition of Eosinophils: Methods and Protocols updates several techniques from the first edition together with novel in vitro and in vivo-based methodologies. Written by internationally recognized expert authorities, this volume provides vital techniques from eosinophil purification to experimental modelling, with each technique spelled out in clear and straightforward terms, assuming no previous knowledge of the method and without necessitating sourcing additional information elsewhere. Written in the highly successful Methods in Molecular Biology format, chapters include introductions to their respective topics, lists of the essential materials and reagents, step-by-step, readily reproducible laboratory protocols, with tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Eosinophils: Methods and Protocols, Second Edition provides the practical means to extend our knowledge of eosinophil function in health and disease, underpinning research that may in turn lead to new hypotheses for future examinations into the role of this intriguing and enigmatic leukocyte. Chapters 10 is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

In Eosinophil: Methods and Protocols, experts in the field of eosinophil biology comprehensively provide detailed methodological insight into the study of this fascinating cell. This book is aimed at a diverse range of basic and clinical scientists who wish to work with eosinophils or who require an update of their knowledge or to gain the information required to study a function of the eosinophil different to their current area of enquiry. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Eosinophil: Methods and Protocols seeks to aid scientist in the discovery of new hypotheses and for further examination of this intriguing cell.

In Eosinophil: Methods and Protocols, experts in the field of eosinophil biology comprehensively provide detailed methodological insight into the study of this fascinating cell. This book is aimed at a diverse range of basic and clinical scientists who wish to work with eosinophils or who require an update of their knowledge or to gain the information required to study a function of the eosinophil different to their current area of enquiry. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Eosinophil: Methods and Protocols seeks to aid scientist in the discovery of new hypotheses and for further examination of this intriguing cell.

This second edition of Eosinophils: Methods and Protocols updates several techniques from the first edition together with novel in vitro and in vivo-based methodologies. Written by internationally recognized expert authorities, this volume provides vital techniques from eosinophil purification to experimental modelling, with each technique spelled out in clear and straightforward terms, assuming no previous knowledge of the method and without necessitating sourcing additional information elsewhere. Written in the highly successful Methods in Molecular Biology format, chapters include introductions to their respective topics, lists of the essential materials and reagents, step-by-step, readily reproducible laboratory protocols, with tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Eosinophils: Methods and Protocols, Second Edition provides the practical means to extend our knowledge of eosinophil function in health and disease, underpinning research that may in turn lead to new hypotheses for future examinations into the role of this intriguing and enigmatic leukocyte. Chapters 10 is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

The eosinophil is an enigmatic cell with a continuing ability to fascinate. In this book, experts in the field of eosinophil biology comprehensively update our knowledge on the human eosinophil in health and disease. Topics discussed include a synopsis of eosinophil characteristics, properties and role in disease. Important information on how eosinophils release their potent and toxic granule proteins will be covered and how these basic proteins give rise to pathologies including issues such as the function of the nerves.