Aim: Keeping in view limitations associated with presently available drugs it is attempting to develop new formulations, which are more effective and less toxic. Keeping above view in mind, the present study was conducted with the following objectives: To find out the antibacterial and anti fungal activity of clove oil. Find out the minimum inhibitory concentration (MIC) of clove oil against Candida albican and E. coli. To find out the efficacy of clove oil against vaginal candidiasis. conclusions: From this study we evaluated that the clove oil was highly effective in reducing the fungal load in vaginal tissues. Thus clove oil being nontoxic quite efficacious may find a place as constituent in the new generation of antifungal drugs."