HPTLC and HPLC are most commonly used chromatographic technique, which is powerful analytical determination and validation of several biomarkers. Results obtained are simple, precise and accurate signifying well quality fingerprint profile of various phytoconsituents as a biomarker obtained from nature. Luteolin is a flavonoid, mostly belongs to class of bioflavonoids and shows antioxidant, anti-inflammatory, and anti-tumor properties. It is found in high amounts in parsley, thyme, peppermint, basil herb, celery and artichoke. It is a well documented biomarker of Elliptica elba. To validate and optimize luteolin from herbal formulation and plant extracts using HPTLC and HPLC, several procedures are well defined and results are well validated.

Nearly everyone has been suffering from liver disorder and disease by higher intake of various medicines, beverages, alcohol, junk food and accidentally inhalation of chemicals, toxins etc. The liver plays a key role in metabolism and clearance of food and drugs which are susceptible to toxicity. Certain medicines, when taken in overdoses and sometimes introduced within therapeutic ranges, may lead to liver toxicity. More than 900 drugs and toxins have been reported to cause liver injury and several herbal remedies are administered to cure liver from these toxicity. Various drugs from plant sources traditionally used to protect liver and given in jaundice in many tribes all over the world. In India, People of Himalayan region traditionally use aerial parts of Roylea elegans (titpati) for protect their liver and crushed leaves are given to infants against jaundice.