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paper he discussed the results whim had been obtained from his
studies of the adrenal gland. The results he obtained are probably
best told in his own words: -"To see whether the adrenal cortex
contains any such reducing agent the gland was cut with a knife
into halves-which were immersed in a 0. 4% AgN0 solution. In a few
minutes the cortical part 3 was seen to darken through the
deposition of reduced metallic silver. In a quarter of an hour the
cut surface of the cortex turned completely black while the medulla
remained largely uncoloured. The black colouration ends towards the
medulla with a sharp margin, which corresponds to the anatomical
margin between the medulla and cortex. Like the medulla, other
organs, such as brain, liver and muscle remain practically
uncoloured. " When the gland was immersed in a mixture of potassium
iodate and acetic acid-a reagent for adrenalin, the medulla became
violet in colour and the cortex remained colourless. SZENT-GYORGYI
therefore came to the conclusion that: -"The adrenal cortex
therefore, contains some specific highly reducing agent which can
be no arte fact, since its action is produced in the almost fresh
material. " Methyl alcohol extracts of the cortex reduced neutral
silver nitrate almost instantaneously and when the portions of the
interrenal glands of Elasmobranm fishes were treated with silver
nitrate solutions they too went black-so that it appeared that this
highly reducing factor was a funda mental constituent of adrenal
tissue."
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