Due to various special physiological features and a genome that greatly differs in structure, gene content and organization from other yeasts, Y. lipolytica is widely used as a host system. With its characteristics, such as the ability to grow on lipids or grease, to accumulate oil and the high capacity for secretion of proteases and lipases, the yeast is of great interest for biotechnological applications. The main topics covered in this Microbiology Monograph are: expression and secretion of heterologous proteins; acid and alkaline extracellular proteases; genetics, production, biochemical characterization and biotechnological application of lipases; production and secretion of several organic acids and flagrances; as well as the functional expression of P450 systems and its use in steroid biotransformation.

Due to various special physiological features and a genome that greatly differs in structure, gene content and organization from other yeasts, "Y. lipolytica" is widely used as a model organism. With its characteristics, such as the ability to accumulate oil and the high capacity for secretion of proteases and lipases, the yeast is also of great interest for biotechnological applications.

The main topics covered in this "Microbiology Monograph" are: comparative genomics; mitochondrial genomics and proteomics, including the analysis of the respiratory chain; transposable elements and their activities; non-coding RNA genes, which display a number of unusual and remarkable features compared to other hemiascomycetes; utilization of hydrophobic substrates, of n-alkane and its oxidized derivatives as sources of carbon and energy; ambient pH signalling; comparison of protein families in non-conventional yeasts and S. cerevisiae; and the sulphur metabolism of cheese-ripening yeast.

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Due to various special physiological features and a genome that greatly differs in structure, gene content and organization from other yeasts, Y. lipolytica is widely used as a model organism. With its characteristics, such as the ability to accumulate oil and the high capacity for secretion of proteases and lipases, the yeast is also of great interest for biotechnological applications. The main topics covered in this Microbiology Monograph are: comparative genomics; mitochondrial genomics and proteomics, including the analysis of the respiratory chain; transposable elements and their activities; non-coding RNA genes, which display a number of unusual and remarkable features compared to other hemiascomycetes; utilization of hydrophobic substrates, of n-alkane and its oxidized derivatives as sources of carbon and energy; ambient pH signalling; comparison of protein families in non-conventional yeasts and S. cerevisiae; and the sulphur metabolism of cheese-ripening yeast. .

Most information on yeasts derives from experiments with the conventional yeasts Saccaromyces cerevisiae and Schizossaccharomyces pombe, the complete nuclear and mitochondrial genome of which has also been sequenced. For all other non-conventional yeasts, investigations are in progress and the rapid development of molecular techniques has allowed an insight also into a variety of non-conventional yeasts.In this bench manual, over 70 practical protocols using 15 different non-conventional yeast species and in addition several protocols of general use are described in detail. All of these experiments on the genetics, biochemistry and biotechnology of yeasts have been contributed by renowned laboratories and have been reproduced many times. The reliable protocols are thus ideally suited also for undergraduate and graduate practical courses.

Due to various special physiological features and a genome that greatly differs in structure, gene content and organization from other yeasts, Y. lipolytica is widely used as a host system. With its characteristics, such as the ability to grow on lipids or grease, to accumulate oil and the high capacity for secretion of proteases and lipases, the yeast is of great interest for biotechnological applications. The main topics covered in this Microbiology Monograph are: expression and secretion of heterologous proteins; acid and alkaline extracellular proteases; genetics, production, biochemical characterization and biotechnological application of lipases; production and secretion of several organic acids and flagrances; as well as the functional expression of P450 systems and its use in steroid biotransformation.