This completely revised edition explores novel discoveries in bacterial genomic research, with a focus on technical and computational improvements as well as methods used for bacterial pangenome analysis, which relies on microbiome studies and metagenomic data. Beginning with up-to-date sequencing methods, the book continues with sections covering methods for deep phylogenetic analysis, the role of metagenomic data in understanding the genomics of the many yet uncultured bacteria, progress in genome-to-phenome inference, as well as computational genomic tools. Written for the highly successful Methods in Molecular Biology series, chapters include the type of practical detail necessary for reproducible results in the lab. Authoritative and up-to-date, Bacterial Pangenomics: Methods and Protocols, Second Edition serves as an ideal guide for both highly qualified investigators in bacterial genomics and for less experienced researchers, including students and teachers, who could use a reference for approaching genomic analysis and genome data.

This completely revised edition explores novel discoveries in bacterial genomic research, with a focus on technical and computational improvements as well as methods used for bacterial pangenome analysis, which relies on microbiome studies and metagenomic data. Beginning with up-to-date sequencing methods, the book continues with sections covering methods for deep phylogenetic analysis, the role of metagenomic data in understanding the genomics of the many yet uncultured bacteria, progress in genome-to-phenome inference, as well as computational genomic tools. Written for the highly successful Methods in Molecular Biology series, chapters include the type of practical detail necessary for reproducible results in the lab. Authoritative and up-to-date, Bacterial Pangenomics: Methods and Protocols, Second Edition serves as an ideal guide for both highly qualified investigators in bacterial genomics and for less experienced researchers, including students and teachers, who could use a reference for approaching genomic analysis and genome data.

This Festschrift is published in honor of Kim Guldstrand Larsen, one of the earliest precursors of computer science in Denmark, on the occasion of his 60th birthday. During the last three decades, Kim Guldstrand Larsen has given major contributions across a remarkably wide range of topics, including real-time, concurrent, and probabilistic models of computation, logic in computer science, and model checking. Since 1995, he has been one of the prime movers behind the model checking tool for real-time systems UPPAAL, for which he was a co-recipient of the CAV Award in 2013. The Festschrift contains 32 papers that feature the broad range of Kim Guldstrand Larsen's research topics, such as formal languages and automata theory; logic; verification, model checking and testing; algorithmic game theory and mechanism design; semantics and reasoning; real-time and distributed systems; and modeling and simulation.