A collection of readily reproducible methods for the design, preparation, and use of RNAs for silencing gene expression in cells and organisms. The techniques range widely and include methods addressing the biochemical aspects of the silencing machinery, RNA silencing in non-mammalian organisms, and the in vivo delivery of siRNAs and silencing vectors. There are also techniques for designing, preparing, and using RNAs to silence gene expression, for fine-tuning regulation by targeting specific isoforms of a given gene, and for the study and use of microRNAs. The protocols follow the successful Methods in Molecular Biology (TM) series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.

This book explores the process of decision-making around having children in a sample of 115 men, women and couples for whom family formation was a recent past, current or imminent future issue. The discussion is initially focused on the extent to which parenthood was contemplated in late adolescence and during the relationship formation/courtship process, and the process by which family sizes are determined. Decision-making associated with having first, second, third and fourth children is then examined in chapters entitled The First Child; The 'Obligatory' Second Child; The Discretionary Third Child and Fourth Children - Negative Reactions, Practical Issues. Decisions to Have Children in Late 20th and Early 21st Century Australia offers a detailed coverage of a topic with resonances and implications that apply to contemporary cultures all around the world. "

A collection of readily reproducible methods for the design, preparation, and use of RNAs for silencing gene expression in cells and organisms. The techniques range widely and include methods addressing the biochemical aspects of the silencing machinery, RNA silencing in non-mammalian organisms, and the in vivo delivery of siRNAs and silencing vectors. There are also techniques for designing, preparing, and using RNAs to silence gene expression, for fine-tuning regulation by targeting specific isoforms of a given gene, and for the study and use of microRNAs. The protocols follow the successful Methods in Molecular Biology (TM) series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.