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In mammalian cells many physiological processes rely on the
dynamics of the organization of lipids and proteins in biological
membranes. The topics in this volume deal with physicochemical
methods in the study of biomembranes. Some of them have a long and
respectable history in the study of soluble proteins and have only
recently been applied to the study of membranes. Some have tradi
tionally been applied to studies of model systems of lipids of
well-defined com position, as well as to intact membranes. Other
methods, by their very nature, apply to organized bilayers
comprised of both protein and lipid. Van Meer and van Genderen
provide us with an introduction to the field (Chapter I). From
their personal perspective regarding the distribution, trans port,
and sorting of membrane lipids, they formulate a number of
biologically relevant questions and show that the physicochemical
methods described in this book may contribute in great measure to
solving these issues. The methods of analytical ultracentrifugation
have served faithfully for 60 years in the study of water-soluble
proteins. The use of detergent extraction of membrane proteins, and
the manipulation of density with H20/D20 mixtures, has extended
this technique to the study of proteins, and in particular their
interactions, from biological membranes. As described by Morris and
Ralston in Chapter 2, this technique can be used to determine a
number of important properties of proteins.
Intracellular Lipid Distribution, Transport and Sorting: A
Cellbiologist's Need for Physicochemical Information; G. Van Meer,
I.L. Van Genderen Biophysical Characterization of Membrane and
Cytoskeletal Proteins by Sedimentation Analysis; M.B. Morris, G.B.
Ralston Monomolecular Layers in the Study of Biomembranes; R.A.
Demel Differential Scanning and Dynamic Calorimetric Studies of
Cooperative Phase Transitions in Phospholipid Bilayer Membranes; Q.
Ye, R.L. Biltonen Ektacytometry of Red Cells; R.M. Johnson Spin
Label ESR Study of Molecular Dynamics of Lipid/Protein Association
in Membranes; L.I. Horvath NMR Methods for Measuring Membrane
Transport; P.W. Kuchel, et al. Determination of Soluble and
Membrane Protein Structure by Fourier Transform Infrared
Spectroscopy; E. Goormaghtigh, et al. Xray Diffraction on
Biomembranes with Emphasis on Lipid Moiety; P. Laggner 2 additional
articles. Index.
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