Currently "Drosophila" is a dominant experimental model in developmental biology and in gene regulation in eukaryotes. This volume summarizes some thirty years of experience in the handling of "in vitro" cultured "Drosophila" cells. Its main emphasis is on gene transfer methodology, cell responses to heat shock, hormonal regulation of genes, and on the expression and mobility of transposable elements.
Key Features
* Some thirty years of experience in handling "in vitro" cultured "Drosophila" cells
* Cell cultures which provide material for a multiplicity of biochemical approaches
* DNA-mediated gene transfer as an irreplaceable tool for analyzing basic mechanisms of regulation
* "Drosophila" cell lines which qualify them for use in biotechnology

Drosophila Cells in Culture, Second Edition, includes comprehensive coverage of cell lines, methods for creating cell lines, methods for genome engineering, and the use of cell lines for genome wide rNAi screens. This publication summarizes over thirty years of experience in the handling of in vitro cultured Drosophila cells alongside recent methods and functional screens. Early and experienced researchers studying drosophila in developmental biology, genetics, neuroscience, and across the biological and biomedical sciences will benefit from this expert knowledge.