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The rapid progression of genetics and molecular biology has turned
chromosomal engineering from science fiction to reality, with the
successful production of transgenic animals with engineered
chromosomes and chromosomes developed for pharmaceutical protein
production which are now ready for the medical industry. Mammalian
Chromosome Engineering: Methods and Protocols provides the reader
with up-to date information on this rapidly evolving field and
strives to take the reader into the exciting realm of chromosomal
engineering from the basic principles to the practical applications
of these new technologies. The five overview and ten protocol
chapters cover the engineering of chromosomes with extrachromosomal
vectors and transposon systems, the manipulation of naturally
occurred minichromosomes, the generation and engineering of
synthetic artificial chromosomes, and the induced de novo platform
artificial chromosome system. Written in the highly successful
Methods in Molecular Biology (TM) series format, protocols chapters
contain brief introductions to their respective topics, lists of
the necessary materials and reagents, step-by-step, readily
reproducible laboratory protocols, and tips on troubleshooting and
avoiding known pitfalls. Authoritative and cutting-edge, Mammalian
Chromosome Engineering: Methods and Protocols serves as a
bench-side resource for current protocols and aims to help
scientists to explore the many prospects for future research and
vital applications.
The rapid progression of genetics and molecular biology has turned
chromosomal engineering from science fiction to reality, with the
successful production of transgenic animals with engineered
chromosomes and chromosomes developed for pharmaceutical protein
production which are now ready for the medical industry. Mammalian
Chromosome Engineering: Methods and Protocols provides the reader
with up-to date information on this rapidly evolving field and
strives to take the reader into the exciting realm of chromosomal
engineering from the basic principles to the practical applications
of these new technologies. The five overview and ten protocol
chapters cover the engineering of chromosomes with extrachromosomal
vectors and transposon systems, the manipulation of naturally
occurred minichromosomes, the generation and engineering of
synthetic artificial chromosomes, and the induced de novo platform
artificial chromosome system. Written in the highly successful
Methods in Molecular Biology (TM) series format, protocols chapters
contain brief introductions to their respective topics, lists of
the necessary materials and reagents, step-by-step, readily
reproducible laboratory protocols, and tips on troubleshooting and
avoiding known pitfalls. Authoritative and cutting-edge, Mammalian
Chromosome Engineering: Methods and Protocols serves as a
bench-side resource for current protocols and aims to help
scientists to explore the many prospects for future research and
vital applications.
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