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Disturbed Ireland - Being the Letters Written During the Winter of 1880-81 (Hardcover): Bernard H. Becker Disturbed Ireland - Being the Letters Written During the Winter of 1880-81 (Hardcover)
Bernard H. Becker
R845 Discovery Miles 8 450 Ships in 12 - 17 working days
Disturbed Ireland (Hardcover): Bernard H. Becker Disturbed Ireland (Hardcover)
Bernard H. Becker
R1,441 Discovery Miles 14 410 Ships in 12 - 17 working days
Doing Business in the New Latin America - Keys to Profit in America's Next-Door Markets (Hardcover, 2nd edition): Thomas H... Doing Business in the New Latin America - Keys to Profit in America's Next-Door Markets (Hardcover, 2nd edition)
Thomas H Becker
R1,649 Discovery Miles 16 490 Ships in 10 - 15 working days

This hands-on guide teaches executives of small and medium-size U.S. companies how to establish and maintain profitable business in Mexico, Central America, South America, and the Caribbean. Unlike the "old" Latin America, today's Latin America is both readily accessible to smaller North American companies and is being transformed into a bustling business environment. However, for those without a native, in-depth understanding of the emerging changes within today's Latin American marketplace and a grasp of the cultural implications at hand, doing business in Latin America can still be challenging for smaller U.S. exporters and importers. Doing Business in the New Latin America: Keys to Profit in America's Next-Door Markets, Second Edition serves as an insider's travel guide and trader's manual for understanding the region's market environment and best export sales opportunities in each of its countries. It lays the groundwork for finding and developing ideal prospects while avoiding pitfalls and foreigners' faux pas. Part I familiarizes readers with Latin America in general, profiling its nations from a business perspective; Part II explains how Latin American business attitudes developed from a historical perspective. The third section of the text focuses on the all-important art of making—and keeping—the deal.

Die Hunderjahrige Republik - Sociale und Politische Zustande in Veriningten Staaten Nordamerika's (German, Paperback):... Die Hunderjahrige Republik - Sociale und Politische Zustande in Veriningten Staaten Nordamerika's (German, Paperback)
John H. Becker
R591 Discovery Miles 5 910 Ships in 12 - 17 working days
The Market, the State, and the Export-Import Bank of the United States, 1934-2000 (Hardcover): William H. Becker, William M.... The Market, the State, and the Export-Import Bank of the United States, 1934-2000 (Hardcover)
William H. Becker, William M. McClenahan Jr
R3,001 R2,806 Discovery Miles 28 060 Save R195 (6%) Ships in 12 - 17 working days

Based on archival sources, this history of the Export-Import Bank of the United States (Ex-Im), focuses on its role in the growing involvement of the U.S. in the international economy. Over the last two decades the Bank has carried on its Congressionally-mandated mission in an increasingly complicated environment; brought on by changes in private capital markets, Congressional constraints on its budgets, major financial crises in Latin America and Southeast Asia. It has survived despite the latest developments in communications and information technology, and the demands of non-governmental organizations (NGOs) devoted to environmental protection.

Eisenhower and the Cold War Economy (Hardcover): William M. McClenahan, William H. Becker Eisenhower and the Cold War Economy (Hardcover)
William M. McClenahan, William H. Becker
R1,696 Discovery Miles 16 960 Ships in 10 - 15 working days

Throughout his two-term presidency, Dwight D. Eisenhower faced the challenge of managing a period of peacetime prosperity after more than two decades of depression, war, and postwar inflation. The essential issue he addressed was how the country would pay for the deepening Cold War and the extent to which such unprecedented peacetime commitments would affect the United States economy and its institutions.

William M. McClenahan, Jr., and William H. Becker explain how Eisenhower's beliefs and his experiences as a military bureaucrat and wartime and postwar commander shaped his economic policies. They explore the macro- and microeconomic policies his administration employed to finance the Cold War while adapting Republican ideas and Eisenhower's economic principles to new domestic and foreign policy environments. They also detail how Eisenhower worked with new instruments of government policy making, such as the Council of Economic Advisers and a strengthened Federal Reserve Board. In assessing his administration's policies, the authors demonstrate that, rather than focusing overwhelmingly on international political affairs at the expense of economic issues, Eisenhower's policies aimed to preserve and enhance the performance of the American free market system, which he believed was inextricably linked to the successful prosecution of the Cold War. While some of the decisions Eisenhower made did not follow conservative doctrine as closely as many in the Republican Party wanted, this book asserts that his approach to and distrust of partisan politics led to success on many fronts and indeed maintained and buttressed the nation's domestic and international economic health.

An important and original contribution, this examination of the Eisenhower administration's economic policy enriches our understanding of the history of the modern American economy, the presidency, and conservatism in the United States.

The Personality Quiz Book Just for You: Learn All About You! (Paperback): H. Becker The Personality Quiz Book Just for You: Learn All About You! (Paperback)
H. Becker
R221 R186 Discovery Miles 1 860 Save R35 (16%) Ships in 10 - 15 working days
The Market, the State, and the Export-Import Bank of the United States, 1934-2000 (Paperback): William H. Becker, William M.... The Market, the State, and the Export-Import Bank of the United States, 1934-2000 (Paperback)
William H. Becker, William M. McClenahan Jr
R925 Discovery Miles 9 250 Ships in 12 - 17 working days

This is the first history of the Export-Import Bank of the United States (Ex-Im) based on archival sources. As the government's exports credit agency, Ex-Im promotes exports through loans, guarantees and insurance and has had an unusual history as a public institution shaped by market principles. Congress mandated that the Bank only provide credit with a reasonable assurance of repayment. But the rules of the market and the needs of the state conflicted at times. Ex-Im has played a part in all the major events that marked the growing involvement of the United States in the international economy. In the last two decades, the bank has carried on its congressionally mandated mission in an increasingly complicated environment brought on by changes in private capital markets; congressional constraints on its budgets; major financial crises in Latin America and South-East Asia; fast-moving developments in communications and information technology and the demands of non-governmental organisations devoted to environmental protection.

Body Experience - The Subjective Dimension of Psyche and Soma Contributions to Psychosomatic Medicine (Paperback, Softcover... Body Experience - The Subjective Dimension of Psyche and Soma Contributions to Psychosomatic Medicine (Paperback, Softcover reprint of the original 1st ed. 1988)
Elmar Brahler; Contributions by H Appelt, H. Becker, P. Bernhard, D. Bongers, …
R2,790 Discovery Miles 27 900 Ships in 10 - 15 working days

In this book body experience is seen as the subjective expression of psyche and soma and is discussed in relation to its significance in modern medical practice and psychoanalysis. The authors relate how the patient's subjective expression of his or her body frequently plays only a marginal role in current therapy and how the central factor of many diseases is consequently missed. Particularly in the growing field of psychoanalytic psychosomatic medicine it will be necessary to pay the issue of body experience more attention. These theoretical and empirical contributions on body experience were specially prepared for the volume. Initial chapters cover a variety of aspects of body experience and its general significance in medicine and psychoanalysis. Following chapters consider body-oriented forms of therapy, sex-related aspects of body experience and the empirical measurement of body experience and bodily complaints.

Diagnostic Imaging in Ophthalmology (Paperback, Softcover reprint of the original 1st ed. 1986): Carlos F. Gonzales, Melvin H.... Diagnostic Imaging in Ophthalmology (Paperback, Softcover reprint of the original 1st ed. 1986)
Carlos F. Gonzales, Melvin H. Becker, Joseph C. Flanagan
R1,564 Discovery Miles 15 640 Ships in 10 - 15 working days

This book has been written for radiologists, ophthalmologists, neurologists, neurosur geons, plastic surgeons, and others interested in the evaluation of disorders with ophthalmologic signs and symptoms. It is designed to provide recent knowledge in this area derived from ultrasonography, computed tomography (CT), and magnetic resonance imaging (MRI). In the past decade, the advent of ultrasonography, computed tomography, and more recently magnetic resonance imaging has provided diagnostic images of the eye, orbit, and brain in a fashion that had been a dream of many prior to the develop ment of these techniques. These newer modes of diagnosis have replaced some previous techniques, such as nuclear medicine imaging and, to some degree, vascular studies and orbitography. There are three sections to this book. The first section is a discussion of the imaging techniques. The second is devoted to the role of these imaging methods in the evaluation of ophthalmic disorders. The last section, dealing with radiotherapy for ophthalmologic tumors, is included because the current imaging techniques are needed for treatment planning. We wish to thank the many people who have assisted us in preparing this manuscript. Among these are many librarians, secretaries, trainees, and photographers. Weare especially indebted to artist Peter Clark for his illustrations and to Mr. Martin Leibo vici, Associate Curator of New York University Medical School and Director of Health Sciences Library of Goldwater Memorial Hospital, New York City. Also, we wish to thank our families for their help and patience."

First Steps in Psychotherapy - Teaching Psychotherapy to Medical Students and General Practitioners (Paperback): H.H. Wolff First Steps in Psychotherapy - Teaching Psychotherapy to Medical Students and General Practitioners (Paperback)
H.H. Wolff; Contributions by H. Becker, I Bloomfield; Edited by W. Knauss; Contributions by W. Brautigam; Edited by …
R1,442 Discovery Miles 14 420 Ships in 10 - 15 working days

The contributors to this volume, like many others concerned with medical education have for a long whilebeen conscious of the fact that in the training of medical students and in the practice of medicine too little attention is being paid to the psychological aspects of illness and to the use of psychotherapy in patient care. In an attempt to fill this gap medical students at University College Hospital, London, have since 1958 been given the opportunity on a voluntary basis to treat a pa tient with weekly analytically-orientated psychotherapy under supervision for a year or longer. In 1977 a similar scheme was started at the Psychosomatic Clinic of Heidel berg University. We were fortunate in obtaining generous financial support from the European Community which has enabled teachers and students from our two uni versities in Great Britain and the Federal Republic of Germany to collaborate in this student-psychotherapy teaching project and to compare our experiences. In this book we present an account of this joint educational endeavour, including our successes and our failures, as well as our attempts to solve some of the problems we have en countered. We decided to let individual supervisors and teachers who inevitably differed in their approach write their own contributions rather than try to present a unified pic ture which would not have given a fair impression of our work and experience. Ifthis has led to some repetition in the text we hope readers will appreciate the reason for it."

The Mechanisms of Atmospheric Oxidation of the Aromatic Hydrocarbons (Hardcover): Jack G. Calvert, Roger Atkinson, Karl H.... The Mechanisms of Atmospheric Oxidation of the Aromatic Hydrocarbons (Hardcover)
Jack G. Calvert, Roger Atkinson, Karl H. Becker, Richard M. Kamens, John H. Seinfeld, …
R3,345 Discovery Miles 33 450 Ships in 12 - 17 working days

A leading cause of polluted air in urban atmospheres is the interaction of sunlight with an air mixture containing nitrogen oxides and reactive hydrocarbons. Several classes of hydrocarbons are highly reactive hydrocarbons; one is the alkenes, the subject of "The Mechanisms of the Atmospheric Oxidation of the Alkenes" (Calvert, et al., OUP, 2000). This book, the second in a series of comprehensive studies of these compounds, will review and evaluate existing literature on the mechanisms of the aromatic hydrocarbons, which have a particularly high potential for ozone generation. The focus of the text will be on problem solving and clarifying uncertain aspects of the chemistry of these compounds.

Carl H. Becker: Beitrage Zur Geschichte AEgyptens Unter Dem Islam. Teil 1 (German, Hardcover, Reprint 2019 ed.): Carl H. Becker Carl H. Becker: Beitrage Zur Geschichte AEgyptens Unter Dem Islam. Teil 1 (German, Hardcover, Reprint 2019 ed.)
Carl H. Becker
R3,383 Discovery Miles 33 830 Ships in 12 - 17 working days

This is a reproduction of a book published before 1923. This book may have occasional imperfections such as missing or blurred pages, poor pictures, errant marks, etc. that were either part of the original artifact, or were introduced by the scanning process. We believe this work is culturally important, and despite the imperfections, have elected to bring it back into print as part of our continuing commitment to the preservation of printed works worldwide. We appreciate your understanding of the imperfections in the preservation process, and hope you enjoy this valuable book.

Beitrage Zur Geschichte AEgyptens Unter Dem Islam (German, Hardcover, Reprint 2019 ed.): Carl H. Becker Beitrage Zur Geschichte AEgyptens Unter Dem Islam (German, Hardcover, Reprint 2019 ed.)
Carl H. Becker
R3,373 Discovery Miles 33 730 Ships in 12 - 17 working days

This is a reproduction of a book published before 1923. This book may have occasional imperfections such as missing or blurred pages, poor pictures, errant marks, etc. that were either part of the original artifact, or were introduced by the scanning process. We believe this work is culturally important, and despite the imperfections, have elected to bring it back into print as part of our continuing commitment to the preservation of printed works worldwide. We appreciate your understanding of the imperfections in the preservation process, and hope you enjoy this valuable book.

Fear of God and the Beginning of Wisdom - The School of Nisibis and the Development of Scholastic Culture in Late Antique... Fear of God and the Beginning of Wisdom - The School of Nisibis and the Development of Scholastic Culture in Late Antique Mesopotamia (Hardcover)
Adam H. Becker
R1,786 Discovery Miles 17 860 Ships in 12 - 17 working days

Fear of God and the Beginning of Wisdom The School of Nisibis and the Development of Scholastic Culture in Late Antique Mesopotamia Adam H. Becker "Adam Becker brings together work in two different linguistic areas, Syriac and Greek, which are usually conducted separately. Since the period dealt with is a time of transition from the ancient to Medieval world, one of immense significance for the subsequent history of both the Middle East and Europe, it is particularly helpful to have a book that shows how these two geographical worlds were intimately linked from a cultural point of view prior to the political separation brought about by the Arab conquests in the seventh century."--Sebastian Brock, Oxford University The School of Nisibis was the main intellectual center of the Church of the East in the sixth and early seventh centuries C.E. and an institution of learning unprecedented in antiquity. "Fear of God and the Beginning of Wisdom" provides a history both of the School and of the scholastic culture of the Church of the East more generally in the late antique and early Islamic periods. Adam H. Becker examines the ideological and intellectual backgrounds of the school movement and reassesses the evidence for the supposed predecessor of the School of Nisibis, the famed School of the Persians of Edessa. Furthermore, he argues that the East-Syrian ("Nestorian") school movement is better understood as an integral and at times contested part of the broader spectrum of East-Syrian monasticism. Becker examines the East-Syrian culture of ritualized learning, which flourished at the same time and in the same place as the famed Babylonian Rabbinic academies. Jews and Christians in Mesopotamia developed similar institutions aimed at inculcating an identity in young males that defined them as beings endowed by their creator with the capacity to study. The East-Syrian schools are the most significant contemporary intellectual institutions immediately comparable to the Rabbinic academies, even as they served as the conduit for the transmission of Greek philosophical texts and ideas to Muslims in the early 'Abbasid period. Adam H. Becker teaches classics and religious studies at New York University. He is coeditor, with Annette Yoshiko Reed, of "The Ways that Never Parted: Jews and Christians from Late Antiquity to the Early Middle Ages." Divinations: Rereading Late Ancient Religion 2006 320 pages 6 x 9 ISBN 978-0-8122-3934-8 Cloth $69.95s 45.50 ISBN 978-0-8122-0120-8 Ebook $69.95s 45.50 World Rights Religion, History Short copy: Since the period dealt with is a time of transition from the ancient to Medieval world, it is particularly helpful to have a book that shows how these two worlds were intimately linked from a cultural point of view prior to the political separation brought about by the Arab conquests in the seventh century."--Sebastian Brock, Oxford University

Praxishandbuch Nachhaltige Produktentwicklung - Ein Leitfaden Mit Tipps Zur Entwicklung Und Vermarktung Nachhaltiger Produkte... Praxishandbuch Nachhaltige Produktentwicklung - Ein Leitfaden Mit Tipps Zur Entwicklung Und Vermarktung Nachhaltiger Produkte (German, Paperback, 1. Aufl. 2018 ed.)
Ulrich Scholz, Sven Pastoors, Joachim H Becker, Daniela Hofmann, Rob Van Dun
R1,831 Discovery Miles 18 310 Ships in 10 - 15 working days

Nachhaltige Lebensmittel, nachhaltige Textilien, nachhaltiger Konsum - immer mehr Unternehmen orientieren sich am Trend und den Grundsatzen der nachhaltigen Entwicklung. Damit bedienen sie nicht nur einen wachsenden Markt, sondern schonen gleichzeitig naturliche Ressourcen und senken so ihre Produktionskosten. Das Praxishandbuch zeigt Unternehmen, wie sie ihre Produktion und ihr Marketing langfristig auf nachhaltige Methoden und Techniken umstellen koennen. Dabei folgen die Autoren dem Green Marketing. Dieser Ansatz geht auf das steigende Umweltbewusstsein der Kunden ein und hilft so Unternehmen, die eigene Marktposition mithilfe nachhaltiger Produkte zu starken. Die Autoren richten sich damit an mittelstandische Unternehmen sowie Entscheider und Fuhrungskrafte in groesseren Unternehmen, die wissen moechten, wie sie mit umweltbewusster Produktentwicklung neue Markte erschliessen und ihre Wettbewerbsfahigkeit steigern koennen. Das Buch ist in vier Themenbloecke zusammengefasst. Dabei geht es sowohl um grundsatzliche Fragen, als auch praktische Fragen: Welche Faktoren begunstigen die Entwicklung nachhaltiger Produkte? Was sind die betrieblichen und rechtlichen Rahmenbedingungen? Was sind die Phasen der nachhaltigen Produktentwicklung? Und wie koennen Unternehmen umweltfreundliche Produkte am besten vermarkten?

Bautechnik (German, Paperback, Softcover reprint of the original 29th ed. 1977): H. Becker Bautechnik (German, Paperback, Softcover reprint of the original 29th ed. 1977)
H. Becker; Assisted by Horst Becker
R1,627 Discovery Miles 16 270 Ships in 10 - 15 working days

innerhalb der Stadte und Ballungsgebiete erforderlich werdenden unterirdischen Verkehrs- wege machen haufig die Anwendung besonderer Baumethoden und dementsprechend den Einsatz spezieller Maschinen notwendig. Die Entwicklung der Mechanisierung auf diesem Teilgebiet des Bauwesens reicht nun auch schon einige Jahrzehnte zuriick. Sowohl die Methoden als auch die Maschinen sind zunachst aus anderen Gebieten, wie z. B. dem Untertagebau oder dem Grundbau, iibernommen worden, bevor sie fiir die speziellen Belange des unterirdischen Stadtebaus weiterentwickelt wurden. An dieser Stelle sind insbesondere der Bau von U-Bahnen [H 26] und von StraBen- tunneln zu nennen, die im Hinblick auf den baubetrieblichen Ablauf oft sehr ahnlich sind. Wo es die ortlichen Verhaltnisse zulassen, wird auch heute noch in del' offenen Bauweise gearbeitet, die schon in den ersten Jahrzehnten dieses Jahrhunderts beim U-Bahn-Bau angewandt und seitdem in verschiedener Hinsicht weiterentwiekelt worden ist. Um den Oberflachenverkehr moglichst nur kurzzeitig zu unterbrechen, werden bei Anwendung dieser Bauweise die Baugruben nach ihrer Fertigstellung mit Hilfe verschiedener Verbau- method en durch entsprechend tragfahige Konstruktionen abgedeekt, so daB der StraBen- verkehr wahrend der Ausfiihrung der Bauarbeiten - in der Regel nur geringfiigig be- hindert - iiber die Baugruben hinweg flieBen kann. Dieses Verfahren ist jedoch nur im Zuge breiter StraBen anwendbar, wogegen beim Unterfahren von eng bebauten Stadt- gebieten gesehlossene Bauweisen angewandt werden, von denen sich im Laufe der letzten drei Jahrzehnte der Schildvortrieb als besonders giinstig erwiesen hat.

Disturbed Ireland Being the Letters Written During the Winter of 1880-81 (Paperback): Bernard H. Becker Disturbed Ireland Being the Letters Written During the Winter of 1880-81 (Paperback)
Bernard H. Becker
R367 Discovery Miles 3 670 Ships in 10 - 15 working days
Disturbed Ireland - Being the Letters Written During the Winter of 1880-81 (Paperback): Bernard H. Becker Disturbed Ireland - Being the Letters Written During the Winter of 1880-81 (Paperback)
Bernard H. Becker
R565 Discovery Miles 5 650 Ships in 10 - 15 working days
Disturbed Ireland (Paperback): Bernard H. Becker Disturbed Ireland (Paperback)
Bernard H. Becker
R1,044 Discovery Miles 10 440 Ships in 10 - 15 working days
Hyperprolaktinamie - Prolaktinome (German, Paperback): P.-H. Althoff, B. Barde, H. Becker, J.S.E. Dericks-Tan, E. Jungmann,... Hyperprolaktinamie - Prolaktinome (German, Paperback)
P.-H. Althoff, B. Barde, H. Becker, J.S.E. Dericks-Tan, E. Jungmann, …
R2,192 Discovery Miles 21 920 Ships in 10 - 15 working days

Die HyperprolaktinAmiesyndrome gehAren zu den facettenreichsten endokrinen Erkrankungen. Innerhalb der gynAkologischen Endokrinologie wird die HyperprolaktinAmie als Ursache von ZyklusstArungen bei ca. 15 % der Frauen mit AmenorrhA gefunden. Bei MAnnern gehArt die HyperprolaktinAmie zu den wichtigsten somatischen Ursachen der Impotenz. AuAerdem spielt sie als Begleiterkrankung anderer endokriner StArungen eine Rolle. Das vorliegende Buch gibt eine Aoebersicht A1/4ber die heute akzeptierten theoretischen, klinischen und therapeutischen Konzepte bei der HyperprolaktinAmie. VAllig neu ist dabei die Integration eines Konzeptes A1/4ber psychologische Befunde. Das Buch ist fA1/4r interessierte A"rzte, welche Patienten mit HyperprolaktinAmiesyndromen behandeln, eine unerlAAliche Informationsquelle.

Erste Schritte in Der Psychotherapie - Erfahrungen Von Medizinstudenten Patienten Und AErzten Mit Psychotherapie Michael Balint... Erste Schritte in Der Psychotherapie - Erfahrungen Von Medizinstudenten Patienten Und AErzten Mit Psychotherapie Michael Balint ALS Lehrer (German, Paperback, 1983 ed.)
H. Becker; Edited by W. Brautigam, W. Knauss; Contributions by I Bloomfield, W. Brautigam; Edited by …
R1,564 Discovery Miles 15 640 Ships in 10 - 15 working days

So alt wie die analytisch gepragte Psychotherapie istder Wunsch, sie nicht allein im Rahmen der klassischen Psychoanalyse, sondern auch in der allgemeinarztlichen Praxis zu verwirklichen. In Deutschland waren es Viktor von Weizsacker und Alex- ander Mitscherlich, in England Michael Balint, die sich dafur einsetzten, Psycho- therapie zu einem integrierten Element arztlichen Tuns uberhaupt werden zu lassen. Die spezialisierte Psychotherapie hat sich in Instituten und Fachkrankenhausem in in den letzten Jahrzehnten zu einem machtigen Bauwerk mit vielen Etagen und An- bauten entwickelt. In der arztlichen Praxis ist die Psychotherapie jedoch unterent- wickelt geblieben. Die psychologische Perspektive gerat in der Krankengeschichte wie auch in der Arzt-Patient-Beziehung immer mehr in eine Randstellung. Mit be- rechtigter Kritik hat man von der modernen Medizin als einersprachlosen Medizin gesprochen. Die publizistisch oft beschworene Nutzung der Arzt-Patient-Bezie- hung und einer Integration der Psychotherapie in die Medizin ist vorerst jedenfalls noch eine Utopie, kein verwirklichtes Konzept. Die Verfasser dieses Buches sehen diese Entwicklung mit Sorge und mochten ihr entgegentreten. Seit 1958 versucht man im University College Hospital (UCH) London, diese Lucke auszufullen und Medizinstudenten praktische psychothera- peutische Fahigkeiten zu vermitteln. Seit 1977 macht die Psychosomatische Klinik der Universitat Heidelberg ein ahnliches Unterrichtsangebot. Von diesen Versu- chen und Erfahrungen wird hier berichtet (Kap. 1und 2). Ein Erfahrungsaustausch von Studenten und Hochschullehrern der beiden Universitaten und eine sehr fruchtbare Zusammenarbeit wurde durch die Unterstiitzung der Europaischen Ge- meinschaft von 1977 bis 1982 moglich, In Kap.

Die Hunderjahrige Republik. Sociale und Politische Zustande in Veriningten Staaten Nordamerika's (Hardcover): Friedrich... Die Hunderjahrige Republik. Sociale und Politische Zustande in Veriningten Staaten Nordamerika's (Hardcover)
Friedrich von Hellwald, John H. Becker
R1,053 Discovery Miles 10 530 Ships in 10 - 15 working days
Disturbed Ireland - Being Letters Written During the Winter of 1880-81 (Paperback): Bernard H. Becker Disturbed Ireland - Being Letters Written During the Winter of 1880-81 (Paperback)
Bernard H. Becker
R434 Discovery Miles 4 340 Ships in 10 - 15 working days
Improved Analysis of DNA Short Tandem Repeats With Time-of-Flight Mass Spectrometry (Paperback): Christopher H Becker, U.S.... Improved Analysis of DNA Short Tandem Repeats With Time-of-Flight Mass Spectrometry (Paperback)
Christopher H Becker, U.S. Department of Justice, Office of Justice Programs
R353 Discovery Miles 3 530 Ships in 10 - 15 working days

The advent of DNA typing and its use for human identity testing has revolutionized law enforcement investigations in recent years by allowing forensic laboratories to match suspects with minuscule amounts of biological evidence from a crime scene. Equally important is the use of DNA to exclude suspects who were not involved in a crime or to identify human remains in an accident. The past decade has seen numerous advances in the DNA testing procedures, most notably among them the development of PCR (polymerase chain reaction)-based DNA typing methods. Technologies for measuring DNA variations, both length and sequence polymorphisms, have also advanced rapidly in the past decade. The time needed to determine a sample's DNA profile has dropped from 6-8 weeks to 1-2 days, and with more recent advancements, the time needed to process samples may decrease to as little as a few hours, maybe even a few minutes. Simultaneous with the evolution of DNA markers and technologies embraced by the forensic community has been the acceptance and use of DNA typing information. All 50 States now have laws requiring DNA typing of convicted offenders, typically for violent crimes such as rape or homicide. While the law enforcement community is gearing up to gather millions of DNA samples from convicted felons, the DNA typing technology needs improvement. Large backlogs of samples exist today due to the high cost of performing the DNA testing and limited capabilities in forensic laboratories. A need exists for more rapid and cost-effective methods for high-throughput DNA analysis to process samples currently being gathered for large criminal DNA databases around the world. While larger numbers of samples can be processed by increasing the number of laboratory personnel and instruments, the development of high-throughput DNA processing technologies promises to be more cost effective in the long run, especially for the generation of large DNA databases. GeneTrace Systems, Inc., a small biotechnology company located in Alameda, California, has developed high-throughput DNA analysis capabilities using time-of-flight mass spectrometry coupled with parallel sample preparation on a robotic workstation. The GeneTrace technology allows several thousand samples to be processed daily. DNA samples can be analyzed in seconds, rather than minutes or hours, and with improved accuracy compared with conventional electrophoresis methods. Overall, the mass spectrometry method described in this study is two orders of magnitude faster in sample processing time than conventional techniques. This NIJ project was initiated to adapt the GeneTrace technology to human identity DNA markers commonly used by forensic DNA laboratories, specifically short tandem repeat (STR) markers. An extension of the original grant was submitted in December 1997 to fund the development of single nucleotide polymorphism (SNP) markers from mitochondrial DNA and the Y chromosome. Based on the results obtained in this study, the authors believe mass spectrometry can be a useful and effective means for high-throughput DNA analysis, and that it has the capabilities to meet the needs of the forensic DNA community for offender DNA databases. However, due to limited resources and a perceived difficulty to enter the forensic DNA market, GeneTrace made a business decision to not pursue this market. While the STR milestones on the original grant were met, only the initial milestones were achieved on the SNP portion of the NIJ grant because of the premature termination on the part of GeneTrace. GeneTrace Systems, Inc., developed an integrated high-throughput DNA analysis system involving the use of proprietary chemistry, robotic sample manipulation, and time-of-flight mass spectrometry. The purpose of this NIJ project was to apply the GeneTrace technology to improve the analysis of STR markers commonly used in forensic DNA laboratories.

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