The development of new photochemical tools, some synthesized by chemists and some provided by nature, is rapidly changing the way neurobiological research is performed in the modern laboratory. In "Photosensitive Molecules for Controlling Biological Function," expert researchers in the field examine the most cutting-edge tools currently available. Divided into three sections, this detailed compendium features techniques involving natural photosensitive proteins, caged neurotransmitters, and small molecule photoswitches that bestow light sensitivity on ion channels and receptors. Written for the "Neuromethods" series, this volume features the type of meticulous description and implementation advice that is crucial for getting optimal results in the lab.

Authoritative and practical, "Photosensitive Molecules for Controlling Biological Function" provides an unbiased comparison of the various photochemical tools currently available for controlling neuronal activity in order to aid scientists in the vital goal of choosing the right tools for the right job.

This fully updated edition provides a series of methods for how best to assess functions of histone deacetylases and acetyltransferases. The disease-relevance of dysregulated protein deacetylation by overexpressed or aberrantly activated histone deacetylases has spurred an intense search for novel and improved inhibitors of these enzymes, as reflected in this collection. Expert contributors explore the generation and evaluation of novel histone deacetylase inhibitors and new and improved techniques to assess acetylation-dependent molecular mechanisms in vitro and in vivo. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, HDAC/HAT Function Assessment and Inhibitor Development: Methods and Protocols, Second Edition serves as an ideal guide for researchers seeking to further elucidate this vital area of study.

Leaders in schools, universities, and other organizations are constantly bombarded with ethical dilemmas. They are challenged with diverse student needs; contradictory approaches presented by faculty and staff; rules and regulations that conflict with desired outcomes, and more. To deal with these challenges, this book advocates an inquiry method to respond to those diverse interests, needs, and values in conflict in educational and other organizational settings. The method the authors present seeks to harness democratic practices for engaging in ethical deliberation and conflict resolution. This book provides the foundation for understanding ethical language as well as probing the tensions in problem solving and ethical decision-making. It provides stories and examples that enable readers to understand terms like deontology, utilitarianism, religious attitudes, eco-feminism, and social justice leadership. Readers are encouraged to test that understanding by using an inquiry method for examining cases set in schools, universities, and other settings to encourage creative thinking and ethical leadership.

This detailed collection covers how the biological functions of histone deacetylases (HDACs) and histone acetyltransferases (HATs) can be detected in various experimental settings, both in vivo and in vitro. The book also covers the generation and specificity of deacetylase inhibitors and how such agents can be used to test experimental hypotheses. Written for the popular Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, as well as tips on troubleshooting and avoiding known pitfalls. Comprehensive and practical, HDAC/HAT Function Assessment and Inhibitor Development: Methods and Protocols serves as an ideal guide to this vital area of study.

School leaders are constantly challenged by diverse students and conflicting interests between faculty and staff. They are often called upon to make sense of ethical quagmires, where rules might conflict with desired outcomes or personal values clash with professional obligations. Negotiating these dilemmas can be challenging, but democratic ethics can offer an effective process to work through them. Drawing from the writings of John Dewey, Leading Through the Quagmire advocates his notion that democracy is an appropriate response to the multitude of conflicting interests, needs, and values in educational settings. Moreover, Enomoto and Kramer propose an inquiry method to harness democratic ethics for engaging in fair deliberation and conflict resolution. This book provides the foundation for understanding tensions, as well as the methods and applications to navigate through them. Stories and examples are provided to enable readers to understand such terms as utilitarianism, ethical tensions, religious attitudes, and eco-feminism in meaningful ways.

Throughout the English-speaking world, and in the many other countries where analytic philosophy is studied, Hillel Steiner is esteemed as one of the foremost contemporary political philosophers. This volume is designed as a festschrift for Steiner and as an important collection of philosophical essays in its own right. The editors have assembled a roster of highly distinguished international contributors, all of whom are eager to pay tribute to Steiner by focusing on topics on which he himself has concentrated. Some of the contributors engage directly with Steiner's work, whereas others focus not directly on his writings but instead grapple with issues that have figured prominently therein. Each essay seeks to advance the debates in which Steiner himself has so notably participated. The study concludes with a response by Steiner himself.

Throughout the English-speaking world, and in the many other countries where analytic philosophy is studied, Hillel Steiner is esteemed as one of the foremost contemporary political philosophers. This volume is designed as a festschrift for Steiner and as an important collection of philosophical essays in its own right. The editors have assembled a roster of highly distinguished international contributors, all of whom are eager to pay tribute to Steiner by focusing on topics on which he himself has concentrated. Some of the contributors engage directly with Steiner's work, whereas others focus not directly on his writings but instead grapple with issues that have figured prominently therein. Each essay seeks to advance the debates in which Steiner himself has so notably participated. The study concludes with a response by Steiner himself.

School leaders are constantly challenged by diverse students and conflicting interests between faculty and staff. They are often called upon to make sense of ethical quagmires, where rules might conflict with desired outcomes or personal values clash with professional obligations. Negotiating these dilemmas can be challenging, but democratic ethics can offer an effective process to work through them. Drawing from the writings of John Dewey, Leading Through the Quagmire advocates his notion that democracy is an appropriate response to the multitude of conflicting interests, needs, and values in educational settings. Moreover, Enomoto and Kramer propose an inquiry method to harness democratic ethics for engaging in fair deliberation and conflict resolution. This book provides the foundation for understanding tensions, as well as the methods and applications to navigate through them. Stories and examples are provided to enable readers to understand such terms as utilitarianism, ethical tensions, religious attitudes, and eco-feminism in meaningful ways.

How are law and morality connected, how do they interact, and in what ways are they distinct? In Part I of this book, Matthew Kramer argues that moral principles can enter into the law of any jurisdiction. He contends that legal officials can invoke moral principles as laws for resolving disputes, and that they can also invoke them as threshold tests which ordinary laws must satisfy. In opposition to many other theorists, Kramer argues that these functions of moral principles are consistent with all the essential characteristics of any legal system. Part II reaffirms the legal positivist argument that law and morality are separable, arguing against the position of natural-law theory, which portrays legal requirements as a species of moral requirements. Kramer contends that even though the existence of a legal system in any sizeable society is essential for the realization of fundamental moral values, law is not inherently moral either in its effects or in its motivational underpinnings. In the final part, Kramer contests the widespread view that people whose conduct is meticulously careful cannot be held morally responsible for harmful effects of their actions. Through this argument, he reveals that fault-independent liability is present even more prominently in morality than in the law. Through a variety of arguments, Where Law and Morality Meet highlights both some surprising affinities and some striking divergences between morality and law.

John Locke's labor theory of property is one of the seminal ideas of political philosophy and served to establish its author's reputation as one of the leading social and political thinkers of all time. Through it Locke addressed many of his most pressing concerns, and earned a reputation as an outstanding spokesman for political individualism - a reputation that lingers widely despite some partial challenges that have been raised in recent years. In this major new study Matthew Kramer offers an extensive critique of the labor theory and investigates the consequences of its downfall. With incisive analyses of the merits and failings of many aspects of Locke's political thought, Kramer advances a powerful challenge to Locke's image as an individualist. Employing a rigorously philosophical methodology, but remaining aware of the insights generated by historical approaches to Locke, Kramer concludes that Locke's political vision was in fact profoundly communitarian.

This detailed collection covers how the biological functions of histone deacetylases (HDACs) and histone acetyltransferases (HATs) can be detected in various experimental settings, both in vivo and in vitro. The book also covers the generation and specificity of deacetylase inhibitors and how such agents can be used to test experimental hypotheses. Written for the popular Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, as well as tips on troubleshooting and avoiding known pitfalls. Comprehensive and practical, HDAC/HAT Function Assessment and Inhibitor Development: Methods and Protocols serves as an ideal guide to this vital area of study.

Critical Care Neurology, Part I: Neurocritical Care focuses on the care specialists and general neurologists that consult in the ICU and their work with patients in acute, life-threatening situations who are dealing with neurologic or neurosurgical crises emanating from either a preexisting neurologic syndrome or from a new neurologic complication appearing as a result of another medical or surgical critical illness. These two separate clinical situations form the pillars of neurocritical care, hence these practices are addressed via two separate, but closely related, HCN volumes. Chapters in both focus on pathophysiology and management, and are tailored for both general neurologists and active neurocritical specialists, with a specific focus on management over diagnostics. Part I addresses the principles of neurocritical care and the management of various neurologic diseases. Part II addresses the interplay between neurologic complications and the surgical, medical, cardiac, and trauma of critical illnesses that most typically present in the ICU.

During the past several decades, political philosophers have frequently clashed with one another over the question whether governments are morally required to remain neutral among reasonable conceptions of excellence and human flourishing. Whereas the numerous followers of John Rawls (and kindred philosophers such as Ronald Dworkin) have maintained that a requirement of neutrality is indeed incumbent on every system of governance, other philosophers - often designated as 'perfectionists' - have argued against the existence of such a requirement. Liberalism with Excellence enters these debates not by plighting itself unequivocally to one side or the other, but instead by reconceiving each of the sides and thus by redirecting the debates that have occurred between them. On the one hand, the book rejects the requirement of neutrality by contending that certain subsidies for the promotion of excellence in sundry areas of human endeavour can be proper and vital uses of resources by governments. Advocating such departures from the constraint of neutrality, the book presents a version of liberalism that can rightly be classified as 'perfectionist'. On the other hand, the species of perfectionism espoused in Liberalism with Excellence diverges markedly from the theories that have usually been so classified. Indeed, much of the book assails various aspects of those theories. What is more, the aspirational perfectionism elaborated in the closing chapters of the volume is reconcilable in most key respects with a suitably amplified version of Rawlsianism. Hence, by reconceiving both the perfectionist side and the neutralist side of the prevailing disputation, Liberalism with Excellence combines and transforms their respective insights.

John Locke's attempt to justify private property is one of the central elements in his political philosophy. Matthew Kramer's new book explores in depth the Lockean theory of property, along with many other aspects of Locke's political thought. Drawing on the techniques of analytic philosophy, Kramer offers some rigorous and extensive techniques of Locke's arguments. While subsequently investigating the consequences of the shortcomings in Locke's reasoning, Kramer maintains that our understanding of Locke's political vision must change considerably. Kramer's book will be of interest to political philosophers, legal philosophers, and intellectual historians.

Leaders in schools, universities, and other organizations are constantly bombarded with ethical dilemmas. They are challenged with diverse student needs; contradictory approaches presented by faculty and staff; rules and regulations that conflict with desired outcomes, and more. To deal with these challenges, this book advocates an inquiry method to respond to those diverse interests, needs, and values in conflict in educational and other organizational settings. The method the authors present seeks to harness democratic practices for engaging in ethical deliberation and conflict resolution. This book provides the foundation for understanding ethical language as well as probing the tensions in problem solving and ethical decision-making. It provides stories and examples that enable readers to understand terms like deontology, utilitarianism, religious attitudes, eco-feminism, and social justice leadership. Readers are encouraged to test that understanding by using an inquiry method for examining cases set in schools, universities, and other settings to encourage creative thinking and ethical leadership.

Torture and Moral Integrity is about the wrongness of torture and the nature of morality. It discusses multiple types of torture with great philosophical acuity and it seeks to explain why interrogational torture and other types of torture are always and everywhere morally wrong. At the same time, it rigorously plumbs the general structure of morality and the intricacies of moral conflicts and it probes some of the chief grounds for the moral illegitimacy of various modes of conduct. It sophisticatedly defends a deontological conception of morality against some subtle critiques that have been mounted during the past few decades by proponents of consequentialism. The book tackles a concrete moral problem: a problem that has been heatedly debated during recent years in the governmental and military institutions of many countries as well as in academic circles. At the same time it tackles some very abstract issues in moral and political philosophy. Moreover, as becomes apparent at numerous junctures, the abstract ruminations and the concrete prescriptions are closely connected: Kramer's recommendations concerning the legal consequences of the perpetration of torture by public officials or private individuals, for example, are based squarely on his more abstract accounts of the nature of torture and the nature of morality. His philosophical reflections on the structure of morality are the vital background for his approach to torture, and his approach to torture is a natural outgrowth of those philosophical reflections.

For many years, Antony Duff has been one of the world's foremost philosophers of criminal law. This volume collects essays by leading criminal law theorists to explore the principal themes in his work. In a response to the essays, Duff clarifies and develops his position on central problems in criminal law theory.
Some of the essays concentrate on the topic of criminalization. That is, they examine what forms of conduct (including attempts, offensiveness, and negligence) can aptly qualify as criminal offences, and what principled limits, if any, should be placed on the reach of the criminal law. Several of the other essays assess the thesis that punishment is justifiable as a form of communication between offenders and their community. Those essays examine the presuppositions (about the nature and function of community, and about the moral structure of atonement) that must be embraced if communication is to be a primary role for punishment. The remaining essays examine the nature and limits of responsibility in the law, as they engage with philosophical debates over 'moral luck' by investigating the ways in which the law can legitimately hold people responsible for events that were not within their control. These chapters tie the first and third parts of the book together, as they explore the relationship between the principles that determine a person's responsibility and the principles that determine which types of actions can appropriately be criminalized.
Finally, Duff responds with comments that seek to defend and clarify his views while also acknowledging the correctness of some of the critics' objections.

Critical Care Neurology, Part II: Neurology of Critical Illness focuses on the care specialists and general neurologists that consult in the ICU and their work with patients in acute, life-threatening situations who are dealing with neurologic or neurosurgical crises emanating from either a preexisting neurologic syndrome or from a new neurologic complication appearing as a result of another medical or surgical critical illness. These two separate clinical situations form the pillars of neurocritical care, hence these practices are addressed via two separate, but closely related, HCN volumes. Chapters in both focus on pathophysiology and management, and are tailored for both general neurologists and active neurocritical specialists, with a specific focus on management over diagnostics. Part I addresses the principles of neurocritical care and the management of various neurologic diseases. Part II addresses the interplay between neurologic complications and the surgical, medical, cardiac, and trauma of critical illnesses that most typically present in the ICU.

In Defense of Legal Positivism is an uncompromising defence of legal positivism that insists on the separability of law and morality. After distinguishing among three facets of morality, Matthew Kramer explores a variety of ways in which law has been perceived as integrally connected to each of those facets.

The development of new photochemical tools, some synthesized by chemists and some provided by nature, is rapidly changing the way neurobiological research is performed in the modern laboratory. In Photosensitive Molecules for Controlling Biological Function, expert researchers in the field examine the most cutting-edge tools currently available. Divided into three sections, this detailed compendium features techniques involving natural photosensitive proteins, caged neurotransmitters, and small molecule photoswitches that bestow light sensitivity on ion channels and receptors. Written for the Neuromethods series, this volume features the type of meticulous description and implementation advice that is crucial for getting optimal results in the lab. Authoritative and practical, Photosensitive Molecules for Controlling Biological Function provides an unbiased comparison of the various photochemical tools currently available for controlling neuronal activity in order to aid scientists in the vital goal of choosing the right tools for the right job.

How are law and morality connected, how do they interact, and in what ways are they distinct? These questions have been a fundamental concern in the modern analytic philosophy of law. In Where Law and Morality Meet Matthew Kramer reviews the most influential accounts of legal and moral reasoning and presents his own conception of whether moral principles should be incorporated into a concept of law.
In Part One, Kramer argues that moral principles can enter into the law of any jurisdiction. He contends that legal officials can invoke moral principles as laws for resolving disputes, and that they can also invoke them as threshold tests which ordinary laws must satisfy. In opposition to many other theorists, Kramer argues that these functions of moral principles are consistent with the essential characteristics of any legal system.
Part Two reaffirms the legal positivist argument that law and morality are separable, arguing against the position of natural-law theory, which portrays legal requirements as a species of moral requirements. Kramer contends that even though the existence of a legal system in any sizeable society is essential for the realization of fundamental moral values, law is not inherently moral either in its effects or in its motivational underpinnings.
In the final part, Kramer contests the widespread view that people whose conduct is meticulously careful cannot be held morally responsible for harmful effects of their actions. Through this argument, he reveals that fault-independent liability is present even more prominently in morality than in the law.

Freedom of Expression as Self-Restraint provides a novel justificatory foundation for the principle of freedom of expression. As the book argues, such a principle is absolute in that it is exceptionless; it imposes general duties that are binding always and everywere on every system of governance. In addition to injecting a new level of philosophical sophistication into the debates over freedom of expression, the book ties the principle to an ideal of governmental self-restraint, and it shows how that ideal connects to the paramount moral responsibility of every system of governance: the responsibility to bring about the political, social, and economic conditions under which every member of society can be warranted in harbouring an ample sense of self-respect. In short, compliance by a system of governance with the principle of freedom of expression is integral to the fulfilment of that paramount responsibility.

Nautilus Book Awards — Silver Award Winner 2010 had been a very good year for Bruce H. Kramer. But what began as a floppy foot and leg weakness led to a shattering diagnosis: he had amyotrophic lateral sclerosis. ALS is a cruel, unrelenting neurodegenerative disease in which the body’s muscles slowly weaken, including those used to move, swallow, talk, and ultimately breathe. There is no cure: ALS is a death sentence. When death is a constant companion, sitting too closely beside you at the dinner table, coloring your thoughts and feelings and words, your outlook on life is utterly transformed. The perspective and insights offered in We Know How This Ends reveal this daily reality and inspire a way forward for anyone who has suffered major loss and for anyone who surely will. Rather than wallowing in sadness and bitterness, anger and denial, Kramer accepted the crushing diagnosis. The educator and musician recognized that if he wanted a meaningful life, then embracing his imminent death was his only viable option. His decision was the foundation for profound, personal reflection and growth, even as his body weakened, and inspired him to share the lessons he was learning from ALS about how to live as fully as possible, even in the midst of devastating grief. At the time Kramer was diagnosed, broadcast journalist Cathy Wurzer was struggling with her own losses, especially her father’s slow descent into the bewildering world of dementia. Mutual friends put this unlikely pair—journalist and educator—together, and the serendipitous result has been a series of remarkable broadcast conversations, a deep friendship, and now this book. Written with wisdom, genuine humor, and down-to-earth observations, We Know How This Ends is far more than a memoir. It is a dignified, courageous, and unflinching look at how acceptance of loss and inevitable death can lead us all to a more meaningful and fulfilling life.

Nautilus Book Awards - Silver Award Winner 2010 had been a very good year for Bruce H. Kramer. But what began as a floppy foot and leg weakness led to a shattering diagnosis: he had amyotrophic lateral sclerosis. ALS is a cruel, unrelenting neurodegenerative disease in which the body's muscles slowly weaken, including those used to move, swallow, talk, and ultimately breathe. There is no cure: ALS is a death sentence. When death is a constant companion, sitting too closely beside you at the dinner table, coloring your thoughts and feelings and words, your outlook on life is utterly transformed. The perspective and insights offered in We Know How This Ends reveal this daily reality and inspire a way forward for anyone who has suffered major loss and for anyone who surely will. Rather than wallowing in sadness and bitterness, anger and denial, Kramer accepted the crushing diagnosis. The educator and musician recognized that if he wanted a meaningful life, then embracing his imminent death was his only viable option. His decision was the foundation for profound, personal reflection and growth, even as his body weakened, and inspired him to share the lessons he was learning from ALS about how to live as fully as possible, even in the midst of devastating grief. At the time Kramer was diagnosed, broadcast journalist Cathy Wurzer was struggling with her own losses, especially her father's slow descent into the bewildering world of dementia. Mutual friends put this unlikely pair-journalist and educator-together, and the serendipitous result has been a series of remarkable broadcast conversations, a deep friendship, and now this book. Written with wisdom, genuine humor, and down-to-earth observations, We Know How This Ends is far more than a memoir. It is a dignified, courageous, and unflinching look at how acceptance of loss and inevitable death can lead us all to a more meaningful and fulfilling life.