The development of new photochemical tools, some synthesized by chemists and some provided by nature, is rapidly changing the way neurobiological research is performed in the modern laboratory. In "Photosensitive Molecules for Controlling Biological Function," expert researchers in the field examine the most cutting-edge tools currently available. Divided into three sections, this detailed compendium features techniques involving natural photosensitive proteins, caged neurotransmitters, and small molecule photoswitches that bestow light sensitivity on ion channels and receptors. Written for the "Neuromethods" series, this volume features the type of meticulous description and implementation advice that is crucial for getting optimal results in the lab.

Authoritative and practical, "Photosensitive Molecules for Controlling Biological Function" provides an unbiased comparison of the various photochemical tools currently available for controlling neuronal activity in order to aid scientists in the vital goal of choosing the right tools for the right job.

This fully updated edition provides a series of methods for how best to assess functions of histone deacetylases and acetyltransferases. The disease-relevance of dysregulated protein deacetylation by overexpressed or aberrantly activated histone deacetylases has spurred an intense search for novel and improved inhibitors of these enzymes, as reflected in this collection. Expert contributors explore the generation and evaluation of novel histone deacetylase inhibitors and new and improved techniques to assess acetylation-dependent molecular mechanisms in vitro and in vivo. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, HDAC/HAT Function Assessment and Inhibitor Development: Methods and Protocols, Second Edition serves as an ideal guide for researchers seeking to further elucidate this vital area of study.

Leaders in schools, universities, and other organizations are constantly bombarded with ethical dilemmas. They are challenged with diverse student needs; contradictory approaches presented by faculty and staff; rules and regulations that conflict with desired outcomes, and more. To deal with these challenges, this book advocates an inquiry method to respond to those diverse interests, needs, and values in conflict in educational and other organizational settings. The method the authors present seeks to harness democratic practices for engaging in ethical deliberation and conflict resolution. This book provides the foundation for understanding ethical language as well as probing the tensions in problem solving and ethical decision-making. It provides stories and examples that enable readers to understand terms like deontology, utilitarianism, religious attitudes, eco-feminism, and social justice leadership. Readers are encouraged to test that understanding by using an inquiry method for examining cases set in schools, universities, and other settings to encourage creative thinking and ethical leadership.

This detailed collection covers how the biological functions of histone deacetylases (HDACs) and histone acetyltransferases (HATs) can be detected in various experimental settings, both in vivo and in vitro. The book also covers the generation and specificity of deacetylase inhibitors and how such agents can be used to test experimental hypotheses. Written for the popular Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, as well as tips on troubleshooting and avoiding known pitfalls. Comprehensive and practical, HDAC/HAT Function Assessment and Inhibitor Development: Methods and Protocols serves as an ideal guide to this vital area of study.

School leaders are constantly challenged by diverse students and conflicting interests between faculty and staff. They are often called upon to make sense of ethical quagmires, where rules might conflict with desired outcomes or personal values clash with professional obligations. Negotiating these dilemmas can be challenging, but democratic ethics can offer an effective process to work through them. Drawing from the writings of John Dewey, Leading Through the Quagmire advocates his notion that democracy is an appropriate response to the multitude of conflicting interests, needs, and values in educational settings. Moreover, Enomoto and Kramer propose an inquiry method to harness democratic ethics for engaging in fair deliberation and conflict resolution. This book provides the foundation for understanding tensions, as well as the methods and applications to navigate through them. Stories and examples are provided to enable readers to understand such terms as utilitarianism, ethical tensions, religious attitudes, and eco-feminism in meaningful ways.

Throughout the English-speaking world, and in the many other countries where analytic philosophy is studied, Hillel Steiner is esteemed as one of the foremost contemporary political philosophers. This volume is designed as a festschrift for Steiner and as an important collection of philosophical essays in its own right. The editors have assembled a roster of highly distinguished international contributors, all of whom are eager to pay tribute to Steiner by focusing on topics on which he himself has concentrated. Some of the contributors engage directly with Steiner's work, whereas others focus not directly on his writings but instead grapple with issues that have figured prominently therein. Each essay seeks to advance the debates in which Steiner himself has so notably participated. The study concludes with a response by Steiner himself.

Throughout the English-speaking world, and in the many other countries where analytic philosophy is studied, Hillel Steiner is esteemed as one of the foremost contemporary political philosophers. This volume is designed as a festschrift for Steiner and as an important collection of philosophical essays in its own right. The editors have assembled a roster of highly distinguished international contributors, all of whom are eager to pay tribute to Steiner by focusing on topics on which he himself has concentrated. Some of the contributors engage directly with Steiner's work, whereas others focus not directly on his writings but instead grapple with issues that have figured prominently therein. Each essay seeks to advance the debates in which Steiner himself has so notably participated. The study concludes with a response by Steiner himself.

School leaders are constantly challenged by diverse students and conflicting interests between faculty and staff. They are often called upon to make sense of ethical quagmires, where rules might conflict with desired outcomes or personal values clash with professional obligations. Negotiating these dilemmas can be challenging, but democratic ethics can offer an effective process to work through them. Drawing from the writings of John Dewey, Leading Through the Quagmire advocates his notion that democracy is an appropriate response to the multitude of conflicting interests, needs, and values in educational settings. Moreover, Enomoto and Kramer propose an inquiry method to harness democratic ethics for engaging in fair deliberation and conflict resolution. This book provides the foundation for understanding tensions, as well as the methods and applications to navigate through them. Stories and examples are provided to enable readers to understand such terms as utilitarianism, ethical tensions, religious attitudes, and eco-feminism in meaningful ways.

How are law and morality connected, how do they interact, and in what ways are they distinct? In Part I of this book, Matthew Kramer argues that moral principles can enter into the law of any jurisdiction. He contends that legal officials can invoke moral principles as laws for resolving disputes, and that they can also invoke them as threshold tests which ordinary laws must satisfy. In opposition to many other theorists, Kramer argues that these functions of moral principles are consistent with all the essential characteristics of any legal system. Part II reaffirms the legal positivist argument that law and morality are separable, arguing against the position of natural-law theory, which portrays legal requirements as a species of moral requirements. Kramer contends that even though the existence of a legal system in any sizeable society is essential for the realization of fundamental moral values, law is not inherently moral either in its effects or in its motivational underpinnings. In the final part, Kramer contests the widespread view that people whose conduct is meticulously careful cannot be held morally responsible for harmful effects of their actions. Through this argument, he reveals that fault-independent liability is present even more prominently in morality than in the law. Through a variety of arguments, Where Law and Morality Meet highlights both some surprising affinities and some striking divergences between morality and law.

John Locke's attempt to justify private property is one of the central elements in his political philosophy. Matthew Kramer's new book explores in depth the Lockean theory of property, along with many other aspects of Locke's political thought. Drawing on the techniques of analytic philosophy, Kramer offers some rigorous and extensive techniques of Locke's arguments. While subsequently investigating the consequences of the shortcomings in Locke's reasoning, Kramer maintains that our understanding of Locke's political vision must change considerably. Kramer's book will be of interest to political philosophers, legal philosophers, and intellectual historians.

This detailed collection covers how the biological functions of histone deacetylases (HDACs) and histone acetyltransferases (HATs) can be detected in various experimental settings, both in vivo and in vitro. The book also covers the generation and specificity of deacetylase inhibitors and how such agents can be used to test experimental hypotheses. Written for the popular Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, as well as tips on troubleshooting and avoiding known pitfalls. Comprehensive and practical, HDAC/HAT Function Assessment and Inhibitor Development: Methods and Protocols serves as an ideal guide to this vital area of study.

Leaders in schools, universities, and other organizations are constantly bombarded with ethical dilemmas. They are challenged with diverse student needs; contradictory approaches presented by faculty and staff; rules and regulations that conflict with desired outcomes, and more. To deal with these challenges, this book advocates an inquiry method to respond to those diverse interests, needs, and values in conflict in educational and other organizational settings. The method the authors present seeks to harness democratic practices for engaging in ethical deliberation and conflict resolution. This book provides the foundation for understanding ethical language as well as probing the tensions in problem solving and ethical decision-making. It provides stories and examples that enable readers to understand terms like deontology, utilitarianism, religious attitudes, eco-feminism, and social justice leadership. Readers are encouraged to test that understanding by using an inquiry method for examining cases set in schools, universities, and other settings to encourage creative thinking and ethical leadership.

Critical Care Neurology, Part II: Neurology of Critical Illness focuses on the care specialists and general neurologists that consult in the ICU and their work with patients in acute, life-threatening situations who are dealing with neurologic or neurosurgical crises emanating from either a preexisting neurologic syndrome or from a new neurologic complication appearing as a result of another medical or surgical critical illness. These two separate clinical situations form the pillars of neurocritical care, hence these practices are addressed via two separate, but closely related, HCN volumes. Chapters in both focus on pathophysiology and management, and are tailored for both general neurologists and active neurocritical specialists, with a specific focus on management over diagnostics. Part I addresses the principles of neurocritical care and the management of various neurologic diseases. Part II addresses the interplay between neurologic complications and the surgical, medical, cardiac, and trauma of critical illnesses that most typically present in the ICU.

Critical Care Neurology, Part I: Neurocritical Care focuses on the care specialists and general neurologists that consult in the ICU and their work with patients in acute, life-threatening situations who are dealing with neurologic or neurosurgical crises emanating from either a preexisting neurologic syndrome or from a new neurologic complication appearing as a result of another medical or surgical critical illness. These two separate clinical situations form the pillars of neurocritical care, hence these practices are addressed via two separate, but closely related, HCN volumes. Chapters in both focus on pathophysiology and management, and are tailored for both general neurologists and active neurocritical specialists, with a specific focus on management over diagnostics. Part I addresses the principles of neurocritical care and the management of various neurologic diseases. Part II addresses the interplay between neurologic complications and the surgical, medical, cardiac, and trauma of critical illnesses that most typically present in the ICU.

The development of new photochemical tools, some synthesized by chemists and some provided by nature, is rapidly changing the way neurobiological research is performed in the modern laboratory. In Photosensitive Molecules for Controlling Biological Function, expert researchers in the field examine the most cutting-edge tools currently available. Divided into three sections, this detailed compendium features techniques involving natural photosensitive proteins, caged neurotransmitters, and small molecule photoswitches that bestow light sensitivity on ion channels and receptors. Written for the Neuromethods series, this volume features the type of meticulous description and implementation advice that is crucial for getting optimal results in the lab. Authoritative and practical, Photosensitive Molecules for Controlling Biological Function provides an unbiased comparison of the various photochemical tools currently available for controlling neuronal activity in order to aid scientists in the vital goal of choosing the right tools for the right job.

Nautilus Book Awards — Silver Award Winner 2010 had been a very good year for Bruce H. Kramer. But what began as a floppy foot and leg weakness led to a shattering diagnosis: he had amyotrophic lateral sclerosis. ALS is a cruel, unrelenting neurodegenerative disease in which the body’s muscles slowly weaken, including those used to move, swallow, talk, and ultimately breathe. There is no cure: ALS is a death sentence. When death is a constant companion, sitting too closely beside you at the dinner table, coloring your thoughts and feelings and words, your outlook on life is utterly transformed. The perspective and insights offered in We Know How This Ends reveal this daily reality and inspire a way forward for anyone who has suffered major loss and for anyone who surely will. Rather than wallowing in sadness and bitterness, anger and denial, Kramer accepted the crushing diagnosis. The educator and musician recognized that if he wanted a meaningful life, then embracing his imminent death was his only viable option. His decision was the foundation for profound, personal reflection and growth, even as his body weakened, and inspired him to share the lessons he was learning from ALS about how to live as fully as possible, even in the midst of devastating grief. At the time Kramer was diagnosed, broadcast journalist Cathy Wurzer was struggling with her own losses, especially her father’s slow descent into the bewildering world of dementia. Mutual friends put this unlikely pair—journalist and educator—together, and the serendipitous result has been a series of remarkable broadcast conversations, a deep friendship, and now this book. Written with wisdom, genuine humor, and down-to-earth observations, We Know How This Ends is far more than a memoir. It is a dignified, courageous, and unflinching look at how acceptance of loss and inevitable death can lead us all to a more meaningful and fulfilling life.

Nautilus Book Awards - Silver Award Winner 2010 had been a very good year for Bruce H. Kramer. But what began as a floppy foot and leg weakness led to a shattering diagnosis: he had amyotrophic lateral sclerosis. ALS is a cruel, unrelenting neurodegenerative disease in which the body's muscles slowly weaken, including those used to move, swallow, talk, and ultimately breathe. There is no cure: ALS is a death sentence. When death is a constant companion, sitting too closely beside you at the dinner table, coloring your thoughts and feelings and words, your outlook on life is utterly transformed. The perspective and insights offered in We Know How This Ends reveal this daily reality and inspire a way forward for anyone who has suffered major loss and for anyone who surely will. Rather than wallowing in sadness and bitterness, anger and denial, Kramer accepted the crushing diagnosis. The educator and musician recognized that if he wanted a meaningful life, then embracing his imminent death was his only viable option. His decision was the foundation for profound, personal reflection and growth, even as his body weakened, and inspired him to share the lessons he was learning from ALS about how to live as fully as possible, even in the midst of devastating grief. At the time Kramer was diagnosed, broadcast journalist Cathy Wurzer was struggling with her own losses, especially her father's slow descent into the bewildering world of dementia. Mutual friends put this unlikely pair-journalist and educator-together, and the serendipitous result has been a series of remarkable broadcast conversations, a deep friendship, and now this book. Written with wisdom, genuine humor, and down-to-earth observations, We Know How This Ends is far more than a memoir. It is a dignified, courageous, and unflinching look at how acceptance of loss and inevitable death can lead us all to a more meaningful and fulfilling life.

During the past several decades, political philosophers have frequently clashed with one another over the question whether governments are morally required to remain neutral among reasonable conceptions of excellence and human flourishing. Whereas the numerous followers of John Rawls (and kindred philosophers such as Ronald Dworkin) have maintained that a requirement of neutrality is indeed incumbent on every system of governance, other philosophers - often designated as 'perfectionists' - have argued against the existence of such a requirement. Liberalism with Excellence enters these debates not by plighting itself unequivocally to one side or the other, but instead by reconceiving each of the sides and thus by redirecting the debates that have occurred between them. On the one hand, the book rejects the requirement of neutrality by contending that certain subsidies for the promotion of excellence in sundry areas of human endeavour can be proper and vital uses of resources by governments. Advocating such departures from the constraint of neutrality, the book presents a version of liberalism that can rightly be classified as 'perfectionist'. On the other hand, the species of perfectionism espoused in Liberalism with Excellence diverges markedly from the theories that have usually been so classified. Indeed, much of the book assails various aspects of those theories. What is more, the aspirational perfectionism elaborated in the closing chapters of the volume is reconcilable in most key respects with a suitably amplified version of Rawlsianism. Hence, by reconceiving both the perfectionist side and the neutralist side of the prevailing disputation, Liberalism with Excellence combines and transforms their respective insights.

An ecological assessment of natural resource conditions in and adjacent to Redwood National and State Parks, Whiskeytown National Recreation Area, and Oregon Caves National Monument was conducted with the following objectives: 1) provide an initial, science-based evaluation of resource condition status of the Parks and include the associated data used for the evaluation; 2) provide a report that allows Park superintendents and managers to meet Government Performance Results Act and Office of Management and Budget reporting requirements; and 3) develop a reproducible framework for the assessment that can be used at other park units.