The Zebrafish: Disease Models and Chemical Screens, Fourth Edition, the latest volume in the Methods in Cell Biology series, looks at methods for analyzing genetics, genomics, and transcriptomics of zebrafish. Chapters cover such topics as gene-trap mutagenesis, genetic screens for mutations, gene editing in zebrafish, homologous gene targeting, genome-wide RNA tomography, developmental epigenetics, and the zebrafish interactome. * Covers sections on model systems and functional studies, imaging-based approaches, and emerging studies* Includes chapters written by experts in the field* Contains cutting-edge material on the topics discussed

This volume of "Methods in Cell Biology" is the 3e, and provides comprehensive compendia of laboratory protocols and reviews covering all the new methods developed since 2004. This new volume on Disease Models and Chemical Screens, covers two rapidly emerging and compelling applications of the zebrafish.
* Details state-of-the art zebrafish protocols, delineating critical steps in the procedures as well as potential pitfalls
* This volume concentrates on Disease Models and Chemical Screens

The Zebrafish: Genetics, Genomics, and Transcriptomics, Fourth Edition, is the latest volume in the Methods in Cell Biology series that looks at methods for the analysis of genetics, genomics, and transcriptomics of Zebrafish. Chapters cover such topics as gene-trap mutagenesis, genetic Screens for mutations, gene editing in zebrafish, homologous gene targeting, genome-wide RNA tomography, and developmental epigenetics and the zebrafish interactome.

The Zebrafish: Cellular and Developmental Biology, Part B Developmental Biology, the second volume on the topic in the Methods in Cell Biology series, looks at methods for analyzing cellular and developmental biology of zebrafish. Chapters cover such topics as cell biology and developmental and neural biology.

The Zebrafish: Cellular and Developmental Biology, Part A Cellular Biology, is the latest edition in the Methods in Cell Biology series that looks at methods for analyzing cellular and developmental biology of zebrafish. Chapters cover such topics as cell biology and developmental and neural biology.

This volume of "Methods in Cell Biology, " the second of two parts on the subject of zebrafish, provides a comprehensive compendium of laboratory protocols and reviews covering all the new methods developed since 1999.
* Details state-of-the art zebrafish protocols, delineating critical steps in the procedures as well as potential pitfalls
* Illustrates many techiques in full-color
* Summarizes the Zebrafish Genome Project

This volume of Methods in Cell Biology, the first of3 parts on the subject of zebrafish, provides a comprehensive compendia of laboratory protocols and reviews covering all the new methods developed since 2004. This first volume provides state-of-the-art descriptions of novel cellular imaging technologies and methods for culture of zebrafish stem cells, summarizes protocols for analyzingthe development of major organ systems including the central nervous system (CNS), and introduces the use of the zebrafish as a model system for human diseases.
* Details state-of-the art zebrafish protocols, delineating critical steps in the procedures as well as potential pitfalls
* Summarizes the Zebrafish Genome Project"

Due to its prolific reproduction and the external development of the transparent embryo, the zebrafish is the prime model for genetic and developmental studies, as well as research in genomics. While genetically distant from humans, nonetheless the vertebrate zebrafish has comparable organs and tissues that make it the model organism for study of vertebrate development. This book, one of twonew volumes in the "Reliable Lab Solutions "series dealing with zebrafish, brings together a robust and up-to-date collection of time-tested methods presented by the world s leading scientists. Culled from previously published chapters in "Methods in Cell Biology"and updated by the original authorswhere relevant, it providesa comprehensive collection of protocols describing the mostwidely usedtechniques relevant to the study of the cellular and developmental biology of zebrafish. The methodsin thisvolume were hand-selected by the editors, whose goal was to a provide ahandy and cost-effectivecollection offail-safe methods, tips, and tricks of the trade to both experienced researchers and more junior members in the lab.

* Provides busy researchers a quick reference for time-tested methods and protocols that really work, updated where possible by the original authors *Gives pragmatic wisdom to the non-specialist from experts in the field with years of experience with trial and error "

Driven in part by the development of genomics, proteomics, and bioinformatics as new disciplines, there has been a tremendous resurgence of interest in physical methods to investigate macromolecular structure and function in the context of living cells. This volume in "Methods in Cell Biology" is devoted to biophysical techniques "in vivo" and their applications to cellular biology. The volume covers methods-oriented chapters on fundamental as well as cutting-edge techniques in molecular and cellular biophysics. This book is directed toward the broad audience of cell biologists, biophysicists, pharmacologists, and molecular biologists who employ classical and modern biophysical technologies or wish to expand their expertise to include such approaches. It will also interest the biomedical and biotechnology communities for biophysical characterization of drug formulations prior to FDA approval.
* Describes techniques in the context of important biological problems
* Delineates critical steps and potential pitfalls for each method

Driven in part by the development of genomics, proteomics, and bioinformatics as new disciplines, there has been a tremendous resurgence of interest in physical methods to investigate macromolecular structure and function in the context of living cells. This volume in Methods in Cell Biology is devoted to biophysical techniques "in vitro" and their applications to cellular biology. The volume covers methods-oriented chapters on fundamental as well as cutting-edge techniques in molecular and cellular biophysics. This book is directed toward the broad audience of cell biologists, biophysicists, pharmacologists, and molecular biologists who employ classical and modern biophysical technologies or wish to expand their expertise to include such approaches. It will also interest the biomedical and biotechnology communities for biophysical characterization of drug formulations prior to FDA approval.
* Describes techniques in the context of important biological problems
* Delineates critical steps and potential pitfalls for each method
* Includes full-color plates to illustrate techniques

This volume on genetics, genomics, and informatics, will cover new technologies in forward and reverse genetics, transgenesis, the zebrafish genome and mapping technologies, informatics and comparative genomics, and Infrastructure. This volume of "Methods in Cell Biology" will prove valuable both to seasoned zebrafish investigators as well as to those who are newly adopting the zebrafish model as part of their research armamentarium.