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This book has been written to meet the needs of students for
biotechnology courses at various levels of undergraduate and
graduate studies. This book covers all the important aspects of
plant tissue culture viz. nutrition media, micropropagation, organ
culture, cell suspension culture, haploid culture, protoplast
isolation and fusion, secondary metabolite production, somaclonal
variation and cryopreservation. For good understanding of
recombinant DNA technology, chapters on genetic material,
organization of DNA in the genome and basic techniques involved in
recombinant DNA technology have been added. Different aspects on
rDNA technology covered gene cloning, isolation of plant genes,
transposons and gene tagging, in vitro mutagenesis, PCR, molecular
markers and marker assisted selection, gene transfer methods,
chloroplast and mitochondrion DNA transformation, genomics and
bioinformatics. Genomics covers functional and structural genomics,
proteomics, metabolomics, sequencing status of different organisms
and DNA chip technology. Application of biotechnology has been
discussed as transgenics in crop improvement and impact of
recombinant DNA technology mainly in relation to biotech crops.
This book has been written to meet the needs of students for
biotechnology courses at various levels of undergraduate and
graduate studies. This book covers all the important aspects of
plant tissue culture viz. nutrition media, micropropagation, organ
culture, cell suspension culture, haploid culture, protoplast
isolation and fusion, secondary metabolite production, somaclonal
variation and cryopreservation. For good understanding of
recombinant DNA technology, chapters on genetic material,
organization of DNA in the genome and basic techniques involved in
recombinant DNA technology have been added. Different aspects on
rDNA technology covered gene cloning, isolation of plant genes,
transposons and gene tagging, in vitro mutagenesis, PCR, molecular
markers and marker assisted selection, gene transfer methods,
chloroplast and mitochondrion DNA transformation, genomics and
bioinformatics. Genomics covers functional and structural genomics,
proteomics, metabolomics, sequencing status of different organisms
and DNA chip technology. Application of biotechnology has been
discussed as transgenics in crop improvement and impact of
recombinant DNA technology mainly in relation to biotech crops.
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