The success of many biopharmaceuticals relies on the development of stable formulations and/or delivery systems. Biotechnology and Applied Biochemistry serves to facilitate the continued development of these systems to fulfil their commercial potential. With an international research concerning the expression, purification, characterization and application of biological macromolecules in therapeutics and diagnostics.

Drug discovery is a challenging field. It involves team work of several experts including medicinal chemists, natural chemists, pharmacologists, toxicologists, clinicians etc to generate a safe and biologically active drug. In the drug discovery programme, medicinal plants play a key role and are a source of novel molecules. The knowledge of folk medicine and ethnopharmacological studies of plants play a significant role in the development of primary screening methods in drug discovery.Enzymes are the targets for many drugs because of the simplicity of enzyme based assays. It involves the interaction with substrate to form the product. The inhibitor will also interact with the enzyme, hence decreasing the rate of reaction. The enzyme inhibition assays have prompted us to do a primary screening of the methanolic extracts of plants commonly used in Pakistan. Therefore these studies were designed to investigate if any a target drug is present in a plant and to which extent it is there to inhibit a particular enzyme of therapeutic importance.

Diarrhoea represent a major health problem in developing world. It has been estimated that diarrhea claims about two million deaths per year, ranking third among all causes of infectious disease deaths worldwide. Most of these deaths occur in children under five years of age.Eastern medicine is a group of diverse health care systems. There are many Eastern medicines used to treat diarrhea.The information presented here is based on published research, a lot of which is based in developing countries where diarrhea is a serious and common problem.Hence, this book will provide its reader a better understanding about all the aspects of diarrhea particularly its management under the principals of Eastern medicines which are quite life saving and easily understandable

Hyperuricemia is indicative of a high level of uric acid in the blood (>7mg/dl for men, >6mg/dl for women).The hyperuricemia may indicate an increased risk of gout.It is estimated that approximately 15 of every 1,000 males between 35 and 45 years of age have hyperuricemia. Hyperuricemia afflicts an estimated 840 out of 100,000 people. Hyperuricemia can also result in uric acid nephrolithiasis and possible nephropathy if uric acid accumulates in the renal interstitium and tubules.This book comprises of a clinical study conducted for the management of Hyperuricemia in Hamdard University Karachi, Pakistan.Hence it will provide its reader a better understanding of risk factors, causes, clinical features, diagnosis and treatment of Hyperuricemia and its relevant complications with allopathic as well as with modern herbal medicines.

Prevalence of Helicobacter pylori (H. pylori) is very high and well documented in gastroenterological disorders like gastritis, gastric carcinoma, peptic ulcer disease, non ulcer dyspepsia and gastroesophageal reflux disease. More than 50% of the world's population harbor Helicobacter pylori in their upper gastrointestinal tract. It causes a chronic low-level inflammation of the stomach lining and is strongly linked to the development of duodenal and gastric ulcers and stomach cancer. This book will provide the readers a better understanding about the H. pylori related gastroenterological disorders and their proper management with clinical comparative research oriented herbal medicines.