This volume serves as a reference for the dissemination of advances made in the study of Hepatitis B Virus (HBV). Hepatitis B Virus: Methods and Protocols details protocols and techniques ranging from cell culture studies to in vivo and clinical immunology. The chapters in this book discuss treatments of in vitro infection systems, analysis and quantification of cccDNA and its mutations; in vitro polymerase activity assays; cellular trafficking of core proteins; intracellular calcium metabolism; detection, cloning, and sequencing of HBV markers; and new strategies aimed at exploiting new mechanisms for drug discovery. The book also covers classical methods for resolution of extracellular viral particles by native gel electrophoresis, and methods for detecting HBV antigens in drug discovery. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Hepatitis B Virus: Methods and Protocols is a valuable tool for researchers to use toward their advanced studies in HBV.

This book gives an overview on mid-infrared optical glass and fibers laser, it cover the underlying principle, historic background, as well as recent advances in materials processing and enhanced properties for rare earth doped luminescence, spectroscopy lasers, or optical nonlinearity applications. It describes in great detail, the preparation of high purity non-oxide IR glass and fibers to be used as mid-IR fiber laser and supercontinuum sources for optical fiber spectroscopy. It will be useful for academics, researchers and engineers in various disciplines who require a broad introduction to the subject and would like to learn more about the state-of-the-art and upcoming trends in mid-infrared fiber source development, particularly for industrial, medical and military applications.

This book gives an overview on mid-infrared optical glass and fibers laser, it cover the underlying principle, historic background, as well as recent advances in materials processing and enhanced properties for rare earth doped luminescence, spectroscopy lasers, or optical nonlinearity applications. It describes in great detail, the preparation of high purity non-oxide IR glass and fibers to be used as mid-IR fiber laser and supercontinuum sources for optical fiber spectroscopy. It will be useful for academics, researchers and engineers in various disciplines who require a broad introduction to the subject and would like to learn more about the state-of-the-art and upcoming trends in mid-infrared fiber source development, particularly for industrial, medical and military applications.

This volume serves as a reference for the dissemination of advances made in the study of Hepatitis B Virus (HBV). Hepatitis B Virus: Methods and Protocols details protocols and techniques ranging from cell culture studies to in vivo and clinical immunology. The chapters in this book discuss treatments of in vitro infection systems, analysis and quantification of cccDNA and its mutations; in vitro polymerase activity assays; cellular trafficking of core proteins; intracellular calcium metabolism; detection, cloning, and sequencing of HBV markers; and new strategies aimed at exploiting new mechanisms for drug discovery. The book also covers classical methods for resolution of extracellular viral particles by native gel electrophoresis, and methods for detecting HBV antigens in drug discovery. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Hepatitis B Virus: Methods and Protocols is a valuable tool for researchers to use toward their advanced studies in HBV.