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Different sugar types were evaluated on microtuberization
efficiencies of potato and sucrose was considered to be the best
sugar type and 8% sucrose in media was optimized. The media with 5
uM Jasmonic acid resulted the highest number and maximum yield of
microtubers than the other growth regulators used and high nitrate
content media increased microtuber size. Application of 2 mg/L
AgNO3 and propionic acid added media favored the in vitro
microtubers formation. Microtuberization in liquid media followed
by incubation at 8 h photoperiod promoted the number and fresh
weight of microtubers than the other physical supports (agar based,
liquid plus cotton and liquid plus sponge) and incubation (0, 8 and
16 h). The larger microtubers (0.5-0.6 cm diameter) showed
significantly higher survival capacity than smaller ones (0.3-0.4
cm dia). The dormancy termination and sprouting efficiencies of
microtubers were noted best in media with GA3 (100 ppm) treatment,
large size of microtubers and dark incubation. The media with 5 uM
Jasmonic acid showed maximum accumulation of total sugar, reducing
sugar and starch. The vitamin C content of microtubers decreased
with the extended storage periods.
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