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In spite of the wide variety and complexity of biological
materials, nucleic acids are ubiquitous. DNA is becoming the
bioanalyte of choice due to the vast amount of information embedded
in its sequence, its robust chemical nature and the range of highly
sensitive analytical techniques that have been developed. The
results of such analyses can have an important impact on our
society both commercially and in terms of the quality of life.
Absolute confidence in the data generated is therefore of the
utmost importance. This book, produced by LGC as part of the VAM
(Valid Analytical Measurement) Programme, introduces the issues of
validation and quality to the bioanalytical community, specifically
addressing DNA-based analyses. It aims to raise awareness of the
factors that can influence the validity of DNA analysis and the
production of quality data. Emphasis is placed on VAM principles,
as well as additional challenges that are associated with the
analysis of real samples, for example, complex food matrices or
forensic samples that have been subjected to environmental insult.
Information is collated from a variety of sources including
literature, discussions and LGC research, and offers constructive
advice where possible.
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