Recent insight into the transcripts generated from the mammalian genome (i.e. the transcriptome) has revealed that transcription is a far more complex phenomenon than previously thought. In RNA: Methods and Protocols, expert researchers provide the procedures and methods used to describe the structure of messenger RNAs and non-coding RNAs that are transcribed by RNA polymerase II as the immediate gene products in mammalian cells. Focused on the structure of the RNA products of "gene X" and the mapping of proteins associated with these RNAs, the volume presents appropriate information for non-specialists in RNA biology. Written in the highly successful Methods in Molecular Biology (TM) series format, many chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Comprehensive and practical, RNA: Methods and Protocols views the transcriptional landscape with an appreciation for the role that proteins play in the processing and interpretation of genetic information in an attempt to further our crucial knowledge of the many products and sophisticated regulatory networks that result from it.

Even in well-established democratic societies, the political system currently faces a crisis of civic engagement and participation. Increasingly, this lack of engagement and the accompanying erosion of institutional legitimacy result in antidemocratic, populist currents gaining ground. It is an important challenge for both the humanities and the social sciences to analyse this crisis and discuss possible answers that may contribute to strengthening the position of the democratic public sphere in the political process, thus emphasising the crucial role of civic engagement and participation in renewing democracy. The articles in this volume seek the sources for such democratic innovations in a variety of existing, less formalised practices and experiences in public space: in citizens' forums and other concrete arenas for democratic debate and participation in the media in general, including social media and other digital platforms; in social movements; and in artistic interventions in the public sphere. The volume presents selected and edited papers and keynote lectures from the international research conference The Democratic Public Sphere - Current Challenges and Prospects, which was held at Aarhus University on November 5-7, 2015. It includes contributions by keynote speakers Mark E. Warren, Nick Couldry, Donatella della Porta, and Stephen Duncombe.

Recent insight into the transcripts generated from the mammalian genome (i.e. the transcriptome) has revealed that transcription is a far more complex phenomenon than previously thought. In RNA: Methods and Protocols, expert researchers provide the procedures and methods used to describe the structure of messenger RNAs and non-coding RNAs that are transcribed by RNA polymerase II as the immediate gene products in mammalian cells. Focused on the structure of the RNA products of "gene X" and the mapping of proteins associated with these RNAs, the volume presents appropriate information for non-specialists in RNA biology. Written in the highly successful Methods in Molecular Biology (TM) series format, many chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Comprehensive and practical, RNA: Methods and Protocols views the transcriptional landscape with an appreciation for the role that proteins play in the processing and interpretation of genetic information in an attempt to further our crucial knowledge of the many products and sophisticated regulatory networks that result from it.

This volume is co-edited by the director of the Freiburg graduate school "Factual and Fictional Narration" (GRK 1767, Freiburg/Germany) and the director of the Aarhus Centre for Fictionality Studies (University of Aarhus, DK). The collection of essays re-examines the much discussed fact fiction distinction in light of the current burgeoning of research on fictionality. It provides a forum for ongoing work on fictionality from France, Germany and Denmark and Sweden. By placing discussions of the notion of fictionality in one volume, the editors hope to initiate exchange between the different traditions represented in the essays und to help the task of translating the available concepts and terminologies so they can travel between different models and theoretical frameworks.