The book explores the fundamental principles, advances in forensic techniques, and its application on forensic DNA analysis. The book is divided into three modules; the first module provides the historical prospect of forensic DNA typing and introduces fundamentals of forensic DNA typing, methodology, and technical advancements, application of STRs, and DNA databases for forensic DNA profile analysis. Module 2 examines the problems and challenges encountered in extracting DNA and generating DNA profiles. It provides information on the methods and the best practices for DNA isolation from forensic biological samples and human remains like ancient DNA, DNA typing of skeletal remains and disaster victim identification, the importance of DNA typing in human trafficking, and various problems associated with capillary electrophoresis. Module 3 emphasizes various technologies that are based on SNPs, STRs namely Y-STR, X-STR, mitochondrial DNA profiling in forensic science. Module 4 explores the application of non-human forensic DNA typing of domestic animals, wildlife forensics, plant DNA fingerprinting, and microbial forensics. The last module discusses new areas and alternative methods in forensic DNA typing, including Next-Generation Sequencing, and its utility in forensic science, oral microbes, and forensic DNA phenotyping. Given its scope, the book is a useful resource in the field of DNA fingerprinting for scientists, forensic experts, and students at the postgraduate level.

Microorganisms play an important role in the maintenance of the ecosystem structure and function. Bacteria constitute the major part of the microorganisms and possess tremendous potential in many important applications from environmental clean up to the drug discovery. Much advancement has been taken place in the field of research on bacterial systems. This book summarizes the experimental setups required for applied microbiological studies. Important background information, representative results, step by step protocol in this book will be of great use to the students, early career researchers as well as the academicians. The book describes many experiments covering the basic microbiological experiments to the applications of microbial systems for advanced research. Researchers in any field who utilize bacterial systems will find this book very useful. In addition to microbiology and bacteriology, this book will also find useful in molecular biology, genetics, and pathology and the volume should prove to be a valuable laboratory resource in clinical and environmental microbiology, microbial genetics and agricultural research. Unique features * Easy to follow by the users as the experiments have been written in simple language and step-wise manner. * Role of each reagents to be used in each experiment have been described which will help the beginners to understand quickly and design their own experiment. * Each experiment has been equipped with the coloured illustrations for proper understanding of the concept. * Trouble-shootings at the end of each experiment will be helpful in overcoming the problems faced by the users. * Flow-chart of each experiment will quickly guide the users in performing the experiments.

Around the World, metal pollution is a major problem. Conventional practices of toxic metal removal can be ineffective and/or expensive, delaying and exacerbating the crisis. Those communities dealing with contamination must be aware of the fundamentals advances of microbe-mediated metal removal practices because these methods can be easily used and require less remedial intervention. This book describes innovations and efficient applications for metal bioremediation for environments polluted by metal contaminates.

The book explores the fundamental principles, advances in forensic techniques, and its application on forensic DNA analysis. The book is divided into three modules; the first module provides the historical prospect of forensic DNA typing and introduces fundamentals of forensic DNA typing, methodology, and technical advancements, application of STRs, and DNA databases for forensic DNA profile analysis. Module 2 examines the problems and challenges encountered in extracting DNA and generating DNA profiles. It provides information on the methods and the best practices for DNA isolation from forensic biological samples and human remains like ancient DNA, DNA typing of skeletal remains and disaster victim identification, the importance of DNA typing in human trafficking, and various problems associated with capillary electrophoresis. Module 3 emphasizes various technologies that are based on SNPs, STRs namely Y-STR, X-STR, mitochondrial DNA profiling in forensic science. Module 4 explores the application of non-human forensic DNA typing of domestic animals, wildlife forensics, plant DNA fingerprinting, and microbial forensics. The last module discusses new areas and alternative methods in forensic DNA typing, including Next-Generation Sequencing, and its utility in forensic science, oral microbes, and forensic DNA phenotyping. Given its scope, the book is a useful resource in the field of DNA fingerprinting for scientists, forensic experts, and students at the postgraduate level.

Next Generation Sequencing (NGS) Technology in DNA Analysis explains and summarizes next generation sequencing (NGS) technological applications in the field of forensic DNA analysis. The book covers the transition from capillary electrophoresis (CE)-based technique to NGS platforms and the fundamentals of NGS technologies, applications, and advances. Sections provide an overview of NGS technology and forensic science, including information on processing biological samples for forensic analysis, sequence analysis, and data analysis software as well as the analysis of NGS data. The book explores the valuable applications of NGS-based forensic DNA analysis and covers the validations and interpretation guidelines of NGS workflows. With chapter contributions from an international array of experts and the inclusion of practical case studies, this book is a useful reference for academicians and researchers in genetics, biotechnology, bioinformatics, biology, and medicine as well as forensic DNA scientists and practitioners who aim to learn, use, apply, and validate NGS-based technologies.

Microbial and Natural Macromolecules: Synthesis and Applications brings together active scientists and academicians in the field who share updated information and research outcomes from global experts. Microbial macromolecular diversity, molecular composure, genetics, usability of advanced molecular tools and techniques for their study as well as their applicability are discussed with detailed research perspectives.

Microbial Diversity in the Genomic Era: Functional Diversity and Community Analysis, Second Edition presents techniques used for microbial taxonomy and phylogeny, along with their applications and respective strengths and challenges. The book incorporates recently developed biosystematics methods and approaches to assess microbial taxonomy, with suitable recommendations for where to apply them across the range of bacterial identification and infectious disease research. Sections provide a broad overview of microbial genomics research and microbiome directed medicine and update on molecular tools for microbial diversity research, extremophilic microbial diversity, functional microbial diversity across application areas, microbial diversity and infectious disease research, and future directions. Step-by-step methodologies are provided for key techniques, along with applied case studies that break down recent research studies into practical components, thus illuminating pathways for new studies across the field.

Microbial Diversity in the Genomic Era presents insights on the techniques used for microbial taxonomy and phylogeny, along with their applications and respective pros and cons. Though many advanced techniques for the identification of any unknown bacterium are available in the genomics era, a far fewer number of the total microbial species have been discovered and identified to date. The assessment of microbial taxonomy and biosystematics techniques discovered and practiced in the current genomics era with suitable recommendations is the prime focus of this book.

Microbial Biodegradation and Bioremediation: Techniques and Case Studies for Environmental Pollution, Second Edition describes the successful application of microbes and their derivatives for bioremediation of potentially toxic and relatively novel compounds in the environment. Our natural biodiversity and environment is in danger due to the release of continuously emerging potential pollutants by anthropogenic activities. Though many attempts have been made to eradicate and remediate these noxious elements, thousands of xenobiotics of relatively new entities emerge every day, thus worsening the situation. Primitive microorganisms are highly adaptable to toxic environments, and can reduce the load of toxic elements by their successful transformation and remediation. This completely updated new edition presents many new technologies and techniques and includes theoretical context and case studies in every chapter. Microbial Biodegradation and Bioremediation: Techniques and Case Studies for Environmental Pollution, Second Edition serves as a single-source reference and encompasses all categories of pollutants and their applications in a convenient, comprehensive format for researchers in environmental science and engineering, pollution, environmental microbiology, and biotechnology.

Around the World, metal pollution is a major problem. Conventional practices of toxic metal removal can be ineffective and/or expensive, delaying and exacerbating the crisis. Those communities dealing with contamination must be aware of the fundamentals advances of microbe-mediated metal removal practices because these methods can be easily used and require less remedial intervention. This book describes innovations and efficient applications for metal bioremediation for environments polluted by metal contaminates.