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With many recent advances, cancer cell culture research is more
important than ever before. This timely edition of Cancer Cell
Culture: Methods and Protocols covers the basic concepts of cancer
cell biology and culture while expanding upon the recent shift in
cell culture methods from the generation of new cell lines to the
use of primary cells. There are methods to characterize and
authenticate cell lines, to isolate and develop specific types of
cancer cells, and to develop new cell line models. Functional
assays are provided for the evaluation of clonogenicity, cell
proliferation, apoptosis, adhesion, migration, invasion,
senescence, angiogenesis, and cell cycle parameters. Other methods
permit the modification of cells for transfection, drug resistance,
immortalization, and transfer in vivo, the co-culture of different
cell types, and the detection and treatment of contamination. In
this new edition, specific emphasis is placed on safe working
practice for both cells and laboratory researchers. These chapters
contain the information critical to success - only by good practice
and quality control will the results of cancer cell culture
improve. Written in the successful Methods in Molecular Biology
(TM) series format, chapters include introductions to their
respective topics, lists of the necessary materials and reagents,
step-by-step, readily reproducible protocols, and notes on
troubleshooting and avoiding known pitfalls. Authoritative and
accessible, Cancer Cell Culture: Methods and Protocols serves as a
practical guide for scientists of all backgrounds and aims to
convey the appropriate sense of fascination associated with this
research field.
With many recent advances, cancer cell culture research is more
important than ever before. This timely edition of Cancer Cell
Culture: Methods and Protocols covers the basic concepts of cancer
cell biology and culture while expanding upon the recent shift in
cell culture methods from the generation of new cell lines to the
use of primary cells. There are methods to characterize and
authenticate cell lines, to isolate and develop specific types of
cancer cells, and to develop new cell line models. Functional
assays are provided for the evaluation of clonogenicity, cell
proliferation, apoptosis, adhesion, migration, invasion,
senescence, angiogenesis, and cell cycle parameters. Other methods
permit the modification of cells for transfection, drug resistance,
immortalization, and transfer in vivo, the co-culture of different
cell types, and the detection and treatment of contamination. In
this new edition, specific emphasis is placed on safe working
practice for both cells and laboratory researchers. These chapters
contain the information critical to success - only by good practice
and quality control will the results of cancer cell culture
improve. Written in the successful Methods in Molecular Biology
(TM) series format, chapters include introductions to their
respective topics, lists of the necessary materials and reagents,
step-by-step, readily reproducible protocols, and notes on
troubleshooting and avoiding known pitfalls. Authoritative and
accessible, Cancer Cell Culture: Methods and Protocols serves as a
practical guide for scientists of all backgrounds and aims to
convey the appropriate sense of fascination associated with this
research field.
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