This volume compiles new experimental approaches and concepts focusing mostly, but not solely, on ways to manipulate and regulate Ras activity and its downstream signaling output. Chapters detail standard methodologies, biochemical methods, Ras processing trafficking and localization, Ras signaling and inhibition, and in vivo models for studying Ras function. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, application details for both the expert and non-expert reader, and tips on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Ras Activity and Signaling: Methods and Protocols aims to provide support and guidance to lab workers in their work on Ras GTPases and in the design of new projects requiring novel methodologies.

This volume compiles new experimental approaches and concepts focusing mostly, but not solely, on ways to manipulate and regulate Ras activity and its downstream signaling output. Chapters detail standard methodologies, biochemical methods, Ras processing trafficking and localization, Ras signaling and inhibition, and in vivo models for studying Ras function. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, application details for both the expert and non-expert reader, and tips on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Ras Activity and Signaling: Methods and Protocols aims to provide support and guidance to lab workers in their work on Ras GTPases and in the design of new projects requiring novel methodologies.

Ian Prior is one of the most articulate and successful parent-activists in the United States. Armed with 12 inspiring battle-tested rules and revealing stories about sparring with self-righteous Woke neighbours (the "Chardonnay Antifa"), Ian shares secrets about what fellow parents can do in their own school districts to stop the Left's dark vision from taking root. Get ready to laugh. This is not a boring education book. With real world lessons and funny anecdotes readers can connect with, Ian explains how families can win the battle to stop divisive and dangerous concepts rooted in critical race theory and other dangerous left-wing ideologies from spreading to their schools. He convincingly makes the case that the Left envisions a public education system in America where children are no longer the responsibility of their parents but rather mere wards of the state. You'll be shocked and amused by the outrageous stories in Ian's suburban county that unexpectedly became ground zero for the parents' rights movement. This sign-of-the times book helpfully explains the confusing and infuriating cultural moment we find ourselves in. Parents of the World, Unite! is an indispensable book for American families who believe they should be able to raise their children without government interference in their moral, religious, and ethical choices -- and are ready to join a revolution of parents.