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This volume provides state-of-the-art techniques for studying
various aspects of cholesterol homeostasis, including its uptake,
synthesis and efflux from the cell, as well as its trafficking
within the cell. Chapters also cover techniques for studying the
regulation of cholesterol homeostasis at both the transcriptional
and post-translational levels, as well as studying the membrane
topology and structure of cholesterol-related proteins. Written in
the highly successful Methods in Molecular Biology series format,
chapters include introductions to their respective topics, lists of
the necessary materials and reagents, step-by-step, readily
reproducible laboratory protocols, and tips on troubleshooting and
avoiding known pitfalls. Authoritative and practical, Cholesterol
Homeostasis: Methods and Protocols aims to provide key techniques
in tackling the investigation of cholesterol homeostasis.
This volume provides state-of-the-art techniques for studying
various aspects of cholesterol homeostasis, including its uptake,
synthesis and efflux from the cell, as well as its trafficking
within the cell. Chapters also cover techniques for studying the
regulation of cholesterol homeostasis at both the transcriptional
and post-translational levels, as well as studying the membrane
topology and structure of cholesterol-related proteins. Written in
the highly successful Methods in Molecular Biology series format,
chapters include introductions to their respective topics, lists of
the necessary materials and reagents, step-by-step, readily
reproducible laboratory protocols, and tips on troubleshooting and
avoiding known pitfalls. Authoritative and practical, Cholesterol
Homeostasis: Methods and Protocols aims to provide key techniques
in tackling the investigation of cholesterol homeostasis.
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