Arabidopsis Protocols, Third Edition compiles some of the most recent methodologies developed to exploit the Arabidopsis genome. These methodologies cover from the guided access to public resources, to genetic, cell biology, biochemical and physiological techniques, including both those that are widely used as well as those novel techniques likely to open up new avenues of knowledge in the future. In addition, considering the recent unparalleled progress of the "omics" tools in Arabidopsis, leading experts have contributed sections on genome, transcriptome, proteome, metabolome and other whole-system approaches. Arabidopsis thaliana is acknowledged as the most important plant model system by the scientific community and Arabidopsis research has fundamentally influenced our understanding of the basic biology and ecology of plants. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Arabidopsis Protocols, Third Edition seeks to serve both experienced researchers and beginners with its detailed methodologies on this burgeoning scientific field.

For several decades, Arabidopsis thaliana has been the organism of choice in the laboratories of many plant geneticists, physiologists, developmental biologists, and biochemists around the world. During this time, a huge amount of knowledge has been acquired on the biology of this plant species, which has resulted in the development of molecular tools that account for much more efficient research. The significance that Arabidopsis would attain in biological research may have been difficult to foresee in the 1980s, when its use in the laboratory started. In the meantime, it has become the model plant organism, much the same way as Drosophila, Caenorhabditis, or mouse have for animal systems. Today, it is difficult to envision research at the cutting edge of plant biology without the use of Arabidopsis. Since the first edition of Arabidopsis Protocols appeared, new developments have fostered an impressive advance in plant biology that prompted us to prepare Arabidopsis Protocols, Second Edition. Completion of the Arabidopsis genome sequence offered for the first time the opportunity to have in hand all of the genetic information required for studying plant function. In addition, the development of whole systems approaches that allow global analysis of gene expression and protein and metabolite dynamics has encouraged scientists to explore new scenarios that are extending the limits of our knowledge.

This book presents the current knowledge about nonlinear localized travelling excitations in crystals. Excitations can be vibrational, electronic, magnetic or of many other types, in many different types of crystals, as silicates, semiconductors and metals. The book is dedicated to the British scientist FM Russell, recently turned 80. He found 50 years ago that a mineral mica muscovite was able to record elementary charged particles and much later that also some kind of localized excitations, he called them quodons, was also recorded. The tracks, therefore, provide a striking experimental evidence of quodons existence. The first chapter by him presents the state of knowledge in this topic. It is followed by about 18 chapters from world leaders in the field, reviewing different aspects, materials and methods including experiments, molecular dynamics and theory and also presenting the latest results. The last part includes a personal narration of FM Russell of the deciphering of the marks in mica. It provides a unique way to present the science in an accessible way and also illustrates the process of discovery in a scientist's mind.

This book gathers a wealth of research contributions on recent advances in intelligent and distributed computing, and which present both architectural and algorithmic findings in these fields. A major focus is placed on new techniques and applications for evolutionary computation, swarm intelligence, multi-agent systems, multi-criteria optimization and Deep/Shallow machine learning models, all of which are approached as technological drivers to enable autonomous reasoning and decision-making in complex distributed environments. Part of the book is also devoted to new scheduling and resource allocation methods for distributed computing systems. The book represents the peer-reviewed proceedings of the 12th International Symposium on Intelligent Distributed Computing (IDC 2018), which was held in Bilbao, Spain, from October 15 to 17, 2018.

This fourth edition compiles the most recent methodologies developed to exploit the Arabidopsis genome. Chapters detail access to public resources, to genetic, cell biology, biochemical and physiological techniques, and sections on genome, transcriptome, proteome, metabolome and other whole-system approaches. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, application details for both the expert and non-expert reader, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Arabidopsis Protocols, Fourth Edition aims to ensure successful results in the further study of this vital field.

This book proposes a new approach to the study of discourse in documentary film. It considers discourse as a basic factor of translation (as well as contexts, agents, and practices) and draws on the parallels between the disciplines of translating and documentary making to perform a discourse analysis of documentaries centering on migration. By relying on the concept of translation as a heuristic tool, the author highlights the discursive mechanisms of 18 documentaries on Latin American migration shown in the United States by the Public Broadcasting Service series POV between 1996 and 2018. This interdisciplinary approach facilitates a holistic analysis of documentary film discourse, while also raising awareness of positive discourses of migration. The book will be of interest to students and scholars involved in the study of discourse, translation, documentary, television, and migration.

First published in 1999. Routledge is an imprint of Taylor & Francis, an informa company.

Uncover the science of cooking with this International Association of Culinary Professionals Cookbook Award finalist - Molecular Gastronomy: Scientific Cuisine Demystified Molecular Gastronomy: Scientific Cuisine Demystified aims to demystify the intriguing and often mysterious world of cooking that we call molecular gastronomy, or Avangard Nueva Cocina , as Ferran Adria has called it. This book provides readers with crucial knowledge of the ingredients used to execute the fundamental step-by-step techniques provided and is written to help readers expand their skills in the Molecular Gastronomy area. Written by a chef who has spent years cultivating his craft, Molecular Gastronomy: Scientific Cuisine Demystified focuses on introducing the subject to readers and future chefs who have minimal or no experience in the molecular gastronomy of various foods. With its scientific approach, Molecular Gastronomy: Scientific Cuisine Demystified provides a foundation and platform for experimentation, while delving into new and exciting cooking techniques. Stunningly illustrated with hundreds of full-color photos of finished dishes and the process along the way, this unique culinary offering breaks down the science of food while introducing future chefs to some of the most innovative techniques used in today's competitive kitchens.

The radical history of a dynamic, multiracial American neighborhood. "When I think of the future of the United States, and the history that matters in this country, I often think of Boyle Heights."-George J. Sanchez The vision for America's cross-cultural future lies beyond the multicultural myth of the "great melting pot." That idea of diversity often imagined ethnically distinct urban districts-the Little Italys, Koreatowns, and Jewish quarters of American cities-built up over generations and occupying spaces that excluded one another. But the neighborhood of Boyle Heights shows us something altogether different: a dynamic, multiracial community that has forged solidarity through a history of social and political upheaval. Boyle Heights is an in-depth history of the Los Angeles neighborhood, showcasing the potent experiences of its residents, from early contact between Spanish colonizers and native Californians to the internment of Japanese Americans during World War II, the hunt for hidden Communists among the Jewish population, negotiating citizenship and belonging among Latino migrants and Mexican American residents, and beyond. Through each period and every struggle, the residents of Boyle Heights have maintained remarkable solidarity across racial and ethnic lines, acting as a unified polyglot community even as their tribulations have become more explicitly racial in nature. Boyle Heights is immigrant America embodied, and it can serve as the true beacon on a hill toward which the country can strive in a time when racial solidarity and civic resistance have never been in greater need.

This book gathers a wealth of research contributions on recent advances in intelligent and distributed computing, and which present both architectural and algorithmic findings in these fields. A major focus is placed on new techniques and applications for evolutionary computation, swarm intelligence, multi-agent systems, multi-criteria optimization and Deep/Shallow machine learning models, all of which are approached as technological drivers to enable autonomous reasoning and decision-making in complex distributed environments. Part of the book is also devoted to new scheduling and resource allocation methods for distributed computing systems. The book represents the peer-reviewed proceedings of the 12th International Symposium on Intelligent Distributed Computing (IDC 2018), which was held in Bilbao, Spain, from October 15 to 17, 2018.

This book presents the current knowledge about nonlinear localized travelling excitations in crystals. Excitations can be vibrational, electronic, magnetic or of many other types, in many different types of crystals, as silicates, semiconductors and metals. The book is dedicated to the British scientist FM Russell, recently turned 80. He found 50 years ago that a mineral mica muscovite was able to record elementary charged particles and much later that also some kind of localized excitations, he called them quodons, was also recorded. The tracks, therefore, provide a striking experimental evidence of quodons existence. The first chapter by him presents the state of knowledge in this topic. It is followed by about 18 chapters from world leaders in the field, reviewing different aspects, materials and methods including experiments, molecular dynamics and theory and also presenting the latest results. The last part includes a personal narration of FM Russell of the deciphering of the marks in mica. It provides a unique way to present the science in an accessible way and also illustrates the process of discovery in a scientist's mind.

Arabidopsis Protocols, Third Edition compiles some of the most recent methodologies developed to exploit the Arabidopsis genome. These methodologies cover from the guided access to public resources, to genetic, cell biology, biochemical and physiological techniques, including both those that are widely used as well as those novel techniques likely to open up new avenues of knowledge in the future. In addition, considering the recent unparalleled progress of the "omics" tools in Arabidopsis, leading experts have contributed sections on genome, transcriptome, proteome, metabolome and other whole-system approaches. Arabidopsis thaliana is acknowledged as the most important plant model system by the scientific community and Arabidopsis research has fundamentally influenced our understanding of the basic biology and ecology of plants. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Arabidopsis Protocols, Third Edition seeks to serve both experienced researchers and beginners with its detailed methodologies on this burgeoning scientific field.

This fourth edition compiles the most recent methodologies developed to exploit the Arabidopsis genome. Chapters detail access to public resources, to genetic, cell biology, biochemical and physiological techniques, and sections on genome, transcriptome, proteome, metabolome and other whole-system approaches. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, application details for both the expert and non-expert reader, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Arabidopsis Protocols, Fourth Edition aims to ensure successful results in the further study of this vital field.

Twentieth-century Los Angeles has been the locus of one of the most profound and complex interactions between variant cultures in American history. Yet this study is among the first to examine the relationship between ethnicity and identity among the largest immigrant group to that city. By focusing on Mexican immigrants to Los Angeles from 1900 to 1945, George J. Sánchez explores the process by which temporary sojourners altered their orientation to that of permanent residents, thereby laying the foundation for a new Mexican-American culture. Analyzing not only formal programs aimed at these newcomers by the United States and Mexico, but also the world created by these immigrants through family networks, religious practice, musical entertainment, and work and consumption patterns, Sánchez uncovers the creative ways Mexicans adapted their culture to life in the United States. When a formal repatriation campaign pushed thousands to return to Mexico, those remaining in Los Angeles launched new campaigns to gain civil rights as ethnic Americans through labor unions and New Deal politics. The immigrant generation, therefore, laid the groundwork for the emerging Mexican-American identity of their children.

The radical history of a dynamic, multiracial American neighborhood. "When I think of the future of the United States, and the history that matters in this country, I often think of Boyle Heights."-George J. Sanchez The vision for America's cross-cultural future lies beyond the multicultural myth of the "great melting pot." That idea of diversity often imagined ethnically distinct urban districts-the Little Italys, Koreatowns, and Jewish quarters of American cities-built up over generations and occupying spaces that excluded one another. But the neighborhood of Boyle Heights shows us something altogether different: a dynamic, multiracial community that has forged solidarity through a history of social and political upheaval. Boyle Heights is an in-depth history of the Los Angeles neighborhood, showcasing the potent experiences of its residents, from early contact between Spanish colonizers and native Californians to the internment of Japanese Americans during World War II, the hunt for hidden Communists among the Jewish population, negotiating citizenship and belonging among Latino migrants and Mexican American residents, and beyond. Through each period and every struggle, the residents of Boyle Heights have maintained remarkable solidarity across racial and ethnic lines, acting as a unified polyglot community even as their tribulations have become more explicitly racial in nature. Boyle Heights is immigrant America embodied, and it can serve as the true beacon on a hill toward which the country can strive in a time when racial solidarity and civic resistance have never been in greater need.