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Handbook of Flow Cytometry Methods Edited By J. Paul Robinson
Associate Editors: Zbigniew Darzynkiewicz, Phillip Dean, Lynn
Dressler, Hans Tanke and Leon Wheeless Here is the ideal laboratory
manual for investigators involved in research and diagnostic
applications of flow cytometry. Handbook of flow cytometry methods
offers a collection of proven, basic procedures designed for use in
the flow cytometry laboratory. Methods implemented at Purdue
University Cytometry Laboratories and developed in laboratories of
contributors worldwide provide practical, step-by-step procedures
for:
- the preparation and handling of standards, reagents and
specimens
- laboratory analyses for immunophenotyping
- measurement of DNA content and cell cycle analyses
- determination of cell physiology and function
- set up and operation of flow cytometers
- computational and data analysis techniques
- safety precautions
Drawing on their collective experience at leading research centers,
the editors have fashioned a unique "working manual" intended to
serve as the starting point for the evaluation and establishment of
laboratory benchmark standards in flow and image cytometry. Each
method is described in detail to be used and modified depending on
the user's needs. Special features of Handbook of Flow Cytometry
Methods include:
- ample space throughout for personal annotations
- suggestions of alternative procedures; trouble-shooting
tips
- extensive list of reagents and suppliers
- comb-bound format for ease of use at the lab bench
Handbook of Flow Cytometry Methods is an essential laboratory
resource for students, faculty, and researchers in cell and
molecular biology, immunology, cancer biology, hematology/oncology,
and laboratory medicine.
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