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Whether the pursuit is commercially motivated or purely academic,
engineering a novel biological catalyst is an enticing challenge.
High-resolution protein structure analysis allows for rational
alteration of enzyme function, yet many useful enzyme variants are
the product of well-designed selection schemes or screening
strategies. Enzyme Engineering: Methods and Protocols provides
guidance to investigators wishing to create enzyme variants with
desired properties. This detailed volume covers such topics as a
simple method for generating site-specific mutations within
bacterial chromosomes. It also highlights the engineering of two
difference types of rare-cutting endonucleases that show great
potential in gene therapy applications: The newest development is
the emergence of TAL effector nucleases or TALENs. Chapters
describe newly developed technologies in sufficient detail so that
each method can be practiced in a standard molecular biology
laboratory. Written in the successful Methods in Molecular Biology
(TM) series format, chapters include introductions to their
respective topics, lists of the necessary materials and reagents,
step-by-step, readily reproducible protocols, and notes on
troubleshooting and avoiding known pitfalls. Authoritative and
easily accessible Enzyme Engineering: Methods and Protocols will be
valuable for scientists with a budding interest in protein
engineering as well as veterans looking for new approaches to apply
in established discovery programs.
Whether the pursuit is commercially motivated or purely academic,
engineering a novel biological catalyst is an enticing challenge.
High-resolution protein structure analysis allows for rational
alteration of enzyme function, yet many useful enzyme variants are
the product of well-designed selection schemes or screening
strategies. Enzyme Engineering: Methods and Protocols provides
guidance to investigators wishing to create enzyme variants with
desired properties. This detailed volume covers such topics as a
simple method for generating site-specific mutations within
bacterial chromosomes. It also highlights the engineering of two
difference types of rare-cutting endonucleases that show great
potential in gene therapy applications: The newest development is
the emergence of TAL effector nucleases or TALENs. Chapters
describe newly developed technologies in sufficient detail so that
each method can be practiced in a standard molecular biology
laboratory. Written in the successful Methods in Molecular Biology
(TM) series format, chapters include introductions to their
respective topics, lists of the necessary materials and reagents,
step-by-step, readily reproducible protocols, and notes on
troubleshooting and avoiding known pitfalls. Authoritative and
easily accessible Enzyme Engineering: Methods and Protocols will be
valuable for scientists with a budding interest in protein
engineering as well as veterans looking for new approaches to apply
in established discovery programs.
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