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To understand the structures and dynamics of DNA and its interactions with ligands, a complete analysis of its confirmational flexibility is required. This volume and its companion, Volume 212 of Methods in Enzymology, collate in one source all the methods and approaches needed to achieve this goal.
The critically acclaimed laboratory standard, Methods in Enzymology, is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. The series contains much material still relevant today - truly an essential publication for researchers in all fields of life sciences.
Many methods have been developed or adapted for the study of RNA processing, which plays a key role in the expression of genetic information. Volumes 180 and 181 of Methods in Enzymology, RNA Processing, Parts A and B, represent the first comprehensive collation of protocols used for the preparation of substrates, for the preparation of extracts and enzymes, and for the analysis of products in RNA processing. At least half of the chapters present techniques that are directly applicable to other areas of nucleic acid biochemistry and cell biology.
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