This volume presents a collection of methods that have contributed to the current understanding of bacterial persisters. Chapters in the book detail general guidelines for measuring persister levels in bacterial cultures, strategies to enrich and resuscitate persister subpopulations, single-cell approaches for visualizing and characterizing persisters, omics techniques and cellular and animal models for studying persistence. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Bacterial Persistence: Methods and Protocols, Second Edition aims to be a useful practical guide to researchers to help further their study in this field.

This volume presents a collection of methods that have contributed to the current understanding of bacterial persisters. Chapters in the book detail general guidelines for measuring persister levels in bacterial cultures, strategies to enrich and resuscitate persister subpopulations, single-cell approaches for visualizing and characterizing persisters, omics techniques and cellular and animal models for studying persistence. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Bacterial Persistence: Methods and Protocols, Second Edition aims to be a useful practical guide to researchers to help further their study in this field.