This volume describes up-to-date techniques used in understanding the molecular biology of acute myeloid leukemia (AML) and addressing advances in diagnosis, classification, prognostication, and therapeutic strategies to potentially impact overall patient survival. The chapters in this book cover topics such as: cytochemical staining, single-cell mass cytometry of AML and Leukemia stem/progenitor cells, microsphere-based assessment of DNA methylation for AML prognosis, a zebrafish model for evaluating the function of human leukemic gene IDH1 and its mutation, and the isolation of biologically active exosomes from plasma of patients with cancer. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and practical, Acute Myeloid Leukemia: Methods and Protocols is a valuable resource for scientists and researchers to further their studies and advancements in the field of AML.

Principles and Applications of Molecular Diagnostics serves as a comprehensive guide for clinical laboratory professionals applying molecular technology to clinical diagnosis. The first half of the book covers principles and analytical concepts in molecular diagnostics such as genomes and variants, nucleic acids isolation and amplification methods, and measurement techniques, circulating tumor cells, and plasma DNA; the second half presents clinical applications of molecular diagnostics in genetic disease, infectious disease, hematopoietic malignancies, solid tumors, prenatal diagnosis, pharmacogenetics, and identity testing. A thorough yet succinct guide to using molecular testing technology, Principles and Applications of Molecular Diagnostics is an essential resource for laboratory professionals, biologists, chemists, pharmaceutical and biotech researchers, and manufacturers of molecular diagnostics kits and instruments.

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The "Baseball Prospectus" Top 101 Prospects, with complete commentary from "Baseball Prospectus 2014" and dozens of never before published mini-scouting reports by the BP Prospect Team. Top 10 Prospects for every major-league team: 300 prospect capsules, with exclusive updates to account for trades and other player movement since original publication date. Three "Prospects on the Rise" and three "Factors on the Farm" for each major-league system: coverage of an additional 60 prospects at all levels of organized baseball. Get to know some of 2015's top prospects a year before everyone else does. "Baseball Prospectus"' 2014 Organizational Rankings. Every major-league organization, dissected and ranked from top to bottom. The "Baseball Prospectus" fantasy team's Top 100 dynasty league prospects: the guys you need to know to set your dynasty league roster for the rest of the decade. Expanded statistical coverage of all Top 10 Prospects, including advanced historical statistics and 2014 and 2015 PECOTA projections for most prospects.

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