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In the last decade, several different optical imaging techniques,
either based on various voltage or calcium dyes, or more recently
on modified fluorescent or bioluminescent proteins (genetically
encoded) that are sensitive to calcium, have been developed to
study neuronal activity, and especially groups of neurons, with the
goal of mapping and deciphering the neural code underlying major
neurophysiological functions. Genetically Encoded Functional
Indicators brings together expert contributors to present the
development of recent genetic techniques that allow for generating
genetically encoded activity sensors in order to investigate
neuronal activity. Each chapter describes a specific sensor and its
utilization to study neuronal activity in a particular way. Written
in the Neuromethods series style, chapters contain the kind of key
description and implementation advice that guarantees successful
results. Helpful and easy to use, Genetically Encoded Functional
Indicators aims to inspire students and researchers and to serve as
a useful guide to those who wish to start using these different
brain imaging techniques and require a bit of guidance in how best
to choose a technique to match the goal of their study.
In the last decade, several different optical imaging techniques,
either based on various voltage or calcium dyes, or more recently
on modified fluorescent or bioluminescent proteins (genetically
encoded) that are sensitive to calcium, have been developed to
study neuronal activity, and especially groups of neurons, with the
goal of mapping and deciphering the neural code underlying major
neurophysiological functions. Genetically Encoded Functional
Indicators brings together expert contributors to present the
development of recent genetic techniques that allow for generating
genetically encoded activity sensors in order to investigate
neuronal activity. Each chapter describes a specific sensor and its
utilization to study neuronal activity in a particular way. Written
in the Neuromethods series style, chapters contain the kind of key
description and implementation advice that guarantees successful
results. Helpful and easy to use, Genetically Encoded Functional
Indicators aims to inspire students and researchers and to serve as
a useful guide to those who wish to start using these different
brain imaging techniques and require a bit of guidance in how best
to choose a technique to match the goal of their study.
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