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A wealth of information has accumulated over the last few years on
the human genome. The new insights have completely changed the
focus of protein analysis. It is no longer time-consuming analysis
of unknown products, but rather selective identifications of
individual forms, modifications and processings, and overall
analysis of global protein outputs from cells and tissues in health
and disease. This book gears to the rising need of sensitive,
accurate, and fast separation and identification techniques in
proteomics. It discusses current methodologies of modern protein
analysis, from isolation and sample preparation, over analysis and
identification, to final characterization. Several evaluations
concentrate on the now productive approaches of two-dimensional gel
electrophoresis and mass spectrometry, but alternative methods and
further perspectives are also outlined. The book includes an
overlook over current databases to connect protein analysis data
with all available information, ...
Methods in protein sequence analysis constitute important fields in
rapid progress. We have experienced a continuous increase in
analytical sensitivity coupled with decreases in time necessary for
purification and analysis. Several generations of sequencers,
liquid/solid/gas-phase, have passed by and returned in other shapes
during just over two decades. Similarly, the introduction of HPLC
permitted an enormous leap forward in this as in other fields of
biochemistry, and we now start to see new major advances in
purification/analysis through capillary electrophoresis.
Furthermore, progress in the field of mass spectrometry has matched
that in chemical analysis and we witness continuous development,
now emphasizing ion spray and other mass spectrometric approaches.
In short, protein analysis has progressed in line with other
developments in modern science and constitutes an indispensable,
integral part of present-day molecular biology. Even the available
molecular tools, in the form of proteases with different
specificities, have increased in number, although we still have far
to go to reach an array of "restriction proteases" like the sets of
nucleases available to the molecular geneticist. Of course,
conferences have been devoted to protein sequence analysis, in
particular the MPSA (Methods in Protein Sequence Analysis) series,
of which the 8th conference took place in Kiruna, Sweden, July 1-6
1990. Again, we witnessed much progress, saw new instruments, and
experienced further interpretational insights into protein
mechanisms and functions.
The 39 chapters in this volume consider subjects ranging from
genetics, markers, and molecular biology of alcoholism, to clinical
observations and treatment. The aim is to integrate pertinent
information from the fields of molecular and cell biology with view
to establishing a molecular basis of alcohol use and abuse. An
initial preview summarizes historical aspects of alcohol use, and
subsequent chapters concern novel drugs, pharmacological aspects,
gene structures, cloning, and enzymatic properties. Also
contributions by "non-traditional" alcohol scientists have been
included in this collection, in order to highlight possible
interaction and parallels between different fields. Novel results
of particular interest include up-dated summaries on receptors,
enzymes, and other proteins, as well as corresponding gene
structures and regulation, setting the basis for distinguishing
markers and pinpointing further possible pharmacological
treatments.
A wealth of information has accumulated over the last few years on
the human genome. The new insights have completely changed the
focus of protein analysis. It is no longer time-consuming analysis
of unknown products, but rather selective identifications of
individual forms, modifications and processings, and overall
analysis of global protein outputs from cells and tissues in health
and disease. This book gears to the rising need of sensitive,
accurate, and fast separation and identification techniques in
proteomics. It discusses current methodologies of modern protein
analysis, from isolation and sample preparation, over analysis and
identification, to final characterization. Several evaluations
concentrate on the now productive approaches of two-dimensional gel
electrophoresis and mass spectrometry, but alternative methods and
further perspectives are also outlined. The book includes an
overlook over current databases to connect protein analysis data
with all available information, ...
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