This third edition of Electron Microscopy: Methods and Protocols expands upon the previous editions with current, detailed protocols on biological and molecular research techniques based on TEM and SEM as well as other closely related imaging and analytical methods. With new chapters on conventional and microwave assisted specimen, cryo-specimen preparation, negative staining and immunogold labelling techniques, DNA and RNA tracking using hybrization in TEM or Atomic Force Microscopy, TEM crystallography and cryo TEM 3D tomography, 3D tomography of resin embedded tissues using FIB-SEM, Correlative microscopy using fluorescence microscopy, confocal microscopy or immune labelling techniques for both TEM and FIB-SEM and Elemental and isotopic identification and their distribution in cells and tissues using TEM, SEM, Scanning Transmission Electron Microscopy (STEM), Secondary Ion Mass Spectrometry (SIMS) and Nano SIMS. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Electron Microscopy: Methods and Protocols, Third Edition provides the most up-to-date and essential information in electron microscopy techniques and methods provided in this edition will assist in advancing future molecular and biological research.

Chinese hand laundries have been a fixture of America's urban landscape for over one hundred years. Yet little is publicly known about the workings of this familiar institution which originated shortly after Chinese immigrants had started to arrive in some numbers in California in the 1850s. At that time the Chinese worked in a wide range of occupations, hand laundries being one of them. But with the faltering of the Western economy and as European immigration to the United States mounted, the tide of anti-Chinese sentiment swelled, which culminated in violent evictions of the Chinese from West Coast cities and in the imposition of the Chinese Exclusion Act of 1882. The laundry became one of the few occupations in which Chinese were able to continue to work. This book is the definitive scholarly study of Chinese laundries and of those who worked in them in the United States. Sio's work, researched in the 1930s, was completed as a dissertation in 1953 at the University of Chicago's School of Sociology. It is an intimate insider's look at the life and work of Chinese hand laundry workers in Chicago, and is one of the most insightful participant observation studies of this kind. Dr. Siu, himself the son of a laundryman, introduces in it the key sociological concept of the "sojourner" and explores the whole nature of immigrant economies. Considered a classic work by students of overseas Chinese and Asian American studies, The Chinese Laundryman is also a landmark in the study of ethnic occupations and in the social and cultural history of the immigrant in America. Vividly descriptive and highly readable, the book will appeal to anyone interested in the ethnic and the urban experience in America.

This third edition of Electron Microscopy: Methods and Protocols expands upon the previous editions with current, detailed protocols on biological and molecular research techniques based on TEM and SEM as well as other closely related imaging and analytical methods. With new chapters on conventional and microwave assisted specimen, cryo-specimen preparation, negative staining and immunogold labelling techniques, DNA and RNA tracking using hybrization in TEM or Atomic Force Microscopy, TEM crystallography and cryo TEM 3D tomography, 3D tomography of resin embedded tissues using FIB-SEM, Correlative microscopy using fluorescence microscopy, confocal microscopy or immune labelling techniques for both TEM and FIB-SEM and Elemental and isotopic identification and their distribution in cells and tissues using TEM, SEM, Scanning Transmission Electron Microscopy (STEM), Secondary Ion Mass Spectrometry (SIMS) and Nano SIMS. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Electron Microscopy: Methods and Protocols, Third Edition provides the most up-to-date and essential information in electron microscopy techniques and methods provided in this edition will assist in advancing future molecular and biological research.

From George Washington's desire (in the heat of the Revolutionary War) for a proper set of Chinese porcelains for afternoon tea, to the lives of Chinese-Irish couples in the 1830s, to the commercial success of Chang and Eng (the "Siamese Twins"), to rising fears of "heathen Chinee," "New York before Chinatown" offers a provocative look at the role Chinese people, things, and ideas played in the fashioning of American culture and politics.

Piecing together various historical fragments and anecdotes from the years before Chinatown emerged in the late 1870s, historian John Kuo Wei Tchen redraws Manhattan's historical landscape and broadens our understanding of the role of port cultures in the making of American identities. Tchen tells his story in three parts. In the first, he explores America's fascination with Asia as a source of luxury items, cultural taste, and lucrative trade. In the second, he explains how Chinese, European-Americans in Yellowface, and various caricatures became objects of curiosity in the expansive commercial marketplace. In the third part, Tchen focuses on how Americans' attitude toward the Chinese changed from fascination to demonization, leading to the passage of the Chinese Exclusion Acts beginning in 1882.

The "yellow peril" is one of the oldest and most pervasive racist ideas in Western culture-dating back to the birth of European colonialism during the Enlightenment. Yet while Fu Manchu looks almost quaint today, the prejudices that gave him life persist in modern culture. Yellow Peril! is the first comprehensive repository of anti-Asian images and writing, and it surveys the extent of this iniquitous form of paranoia. Written by two dedicated scholars and replete with paintings, photographs, and images drawn from pulp novels, posters, comics, theatrical productions, movies, propagandistic and pseudo-scholarly literature, and a varied world of pop culture ephemera, this is both a unique and fascinating archive and a modern analysis of this crucial historical formation.