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This volume includes chapters on the newest techniques for
measuring metabolites of branched-chain amino acids, many new
enzyme assays such as the measurement of isozymes of
acetohydroxacid synthase, and the purification of recently
described enzymes such as isovaleryl-CoA dehydrogenase.
Volume 324 of Methods in Enzymology supplements Volume 166. It
includes genetic information (cloning, gene expression) and
information on human genetic diseases not available when Volume 166
was published.
General Description of the Series:
The critically acclaimed laboratory standard for more than forty
years, Methods in Enzymology is one of the most highly respected
publications in the field of biochemistry. Since 1955, each volume
has been eagerly awaited, frequently consulted, and praised by
researchers and reviewers alike. Now with more than 300 volumes
(all of them still in print), the series contains much material
still relevant today--truly an essential publication for
researchers in all fields of life sciences.
Key Features
* Preparation of substrates and assay of enzymes
* Cloning, expression, and purification of enzymes
* Detection and consequences of genetic defects
* Regulation and expression of enzymes
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