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Major advances in instrumentation and technology have made
high-performance liquid chromatography (HPLC) a pervasive tool in
virtually all areas of chemical and biomedical research today.
Despite HPLC's enormous growth, technical problems still bedevil
most users at least occasionally. Based on their immensely
successful short courses, Dolan and Snyder's landmark new book
--Troubleshooting LC Systems--now makes available to every
practicing chromatographer the authors' wealth of expertise from
years of hands-on troubleshooting of real-world LC
problems--problems that routinely cause crises and unproductive
downtime in nearly every lab. Believing that successful
troubleshooting requires an understanding of the basics, Dolan and
Snyder open by setting forth all the fundamental principles of LC
operation, then move on to a microscopic description of all the
elements of LC devices, to lay out their optimal preventive
maintenance routines, and ultimately to offer an unprecedentedly
thorough treatment of each and every facet of
troubleshooting.Displaying a special tutorial mastery--honed to a
fineness during their ongoing teaching and consulting activities,
and a well-received feature column in LC Magazine--the book's
chapters cover: automation and robotics * approaches to
troubleshooting * reactions of samples * basic separation
techniques * valves, tubing, syringes * band broadening and tailing
* preventive maintenance * column packing and flushing * individual
LC modules * troubleshooting principles * reagents and buffering *
specific problems in separation * ghost and vacancy peaks *
quantitation and calibration * pumps, seals, and pistons * gradient
elution problems * column composition * concentration and blockages
* sample cleanup * temperature and mixing effects * detectors and
recorders * column packing and flushing. In short, Dolan and
Snyder's eminently practical new guide to Troubleshooting LC
Systems is an essential laboratory handbook--one that empowers
expert and novice chromatographer alike to solve every sort of
practical laboratory problem on a day-to-day basis. No one using an
LC system should be without it
Major advances in instrumentation and technology have made
high-performance liquid chromatography (HPLC) a pervasive tool in
virtually all areas of chemical and biomedical research today.
Despite HPLC's enormous growth, technical problems still bedevil
most users at least occasionally.
Based on their immensely successful short courses, Dolan and
Snyder's landmark new book --Troubleshooting LC Systems--now makes
available to every practicing chromatographer the authors' wealth
of expertise from years of hands-on troubleshooting of real-world
LC problems--problems that routinely cause crises and unproductive
downtime in nearly every lab.
Believing that successful troubleshooting requires an
understanding of the basics, Dolan and Snyder open by setting forth
all the fundamental principles of LC operation, then move on to a
microscopic description of all the elements of LC devices, to lay
out their optimal preventive maintenance routines, and ultimately
to offer an unprecedentedly thorough treatment of each and every
facet of troubleshooting.
Displaying a special tutorial mastery--honed to a fineness during
their ongoing teaching and consulting activities, and a
well-received feature column in LC Magazine--the book's chapters
cover: automation and robotics a [ approaches to troubleshooting a
[ reactions of samples a [ basic separation techniques a [ valves,
tubing, syringes a [ band broadening and tailing a [ preventive
maintenance a [ column packing and flushing a [ individual LC
modules a [ troubleshooting principles a [ reagents and buffering a
[ specific problems in separation a [ ghost and vacancy peaks a [
quantitation and calibration a [ pumps, seals, and pistons a
[gradient elution problems a [ column composition a [ concentration
and blockages a [ sample cleanup a [ temperature and mixing effects
a [ detectors and recorders a [ column packing and flushing.
In short, Dolan and Snyder's eminently practical new guide to
Troubleshooting LC Systems is an essential laboratory handbook--one
that empowers expert and novice chromatographer alike to solve
every sort of practical laboratory problem on a day-to-day basis.
No one using an LC system should be without it!
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