This volume explores the latest techniques used in the field of mammalian germ cell biology to study key experimental approaches and perspectives for critical analysis of spermatogonial subsets including the rare spermatogonial stem cells (SSCs) pool.  Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding common pitfalls. Cutting-edge and comprehensive, Spermatogonial Stem Cells: Methods and Protocols is a valuable resource for both new and experiences investigators looking to expand their knowledge in this developing field.  

This book provides a resource of current understandings about various aspects of the biology of spermatogonia in mammals. Considering that covering the entire gamut of all things spermatogonia is a difficult task, specific topics were selected to provide foundational information that will be useful for seasoned researchers in the field of germ cell biology as well as investigators entering the area. Looking to the future, the editors predict that the foundational information provided in this book -- combined with the advent of new tools and budding interests in use of non-rodent mammalian models -- will produce another major advance in knowledge regarding the biology of spermatogonia over the next decade. In particular, we anticipate that the core molecular machinery driving different spermatogonial states in most, if not all, mammals will be described fully, the extrinsic signals emanating from somatic support cell populations to influence spermatogonial functions will become fully known, and the capacity to derive long-term cultures of SSCs and transplant the population to regenerate spermatogenesis and fertility will become a reality for higher order mammals.