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Fluorescence is a very powerful tool for work at the frontier of
cell biology, photobiology and bioinstrumentation. The stated aim
of the workshop was to highlight the significance of fluorescence
work for the understanding of cell and tissue physiology,
physiopathology and pharmacology, particulary in terms of the
analytical use of fluorescent probes in oncology. In the
organization of the workshop a multidisciplinary approach was
selected. The purpose of the Advanced Research Workshop (ARW) was
to bring together researchers in the various disciplines of tissue
optics, imaging, microspectrofluorometry and state of the art
probes, in order to explore the full benefits that can be derived
in biomedicine through the convergence of these approaches. When
applied to in vivo and in situ studies, fluorescence and related
optical methods enable us to explore within tissues, cells and
organelles photon effects previously understood only in solution
photochemistry. Processes which can be studied at the molecular
level by photophysics, photochemistry and physical chemistry can be
evaluated in living tissue by fluorescence spectroscopy and imaging
at the intracellular level in terms of structure and function.
Thus, fluorescence adds a new dimension to cell biology and
physiology. This approach is now supported by a full and versatile,
rapidly growing armamentarium of new selective probes for
organelles, enzymes, cations, cytoskeleton and metabolic control.
Fluorescence is a very powerful tool for work at the frontier of
cell biology, photobiology and bioinstrumentation. The stated aim
of the workshop was to highlight the significance of fluorescence
work for the understanding of cell and tissue physiology,
physiopathology and pharmacology, particulary in terms of the
analytical use of fluorescent probes in oncology. In the
organization of the workshop a multidisciplinary approach was
selected. The purpose of the Advanced Research Workshop (ARW) was
to bring together researchers in the various disciplines of tissue
optics, imaging, microspectrofluorometry and state of the art
probes, in order to explore the full benefits that can be derived
in biomedicine through the convergence of these approaches. When
applied to in vivo and in situ studies, fluorescence and related
optical methods enable us to explore within tissues, cells and
organelles photon effects previously understood only in solution
photochemistry. Processes which can be studied at the molecular
level by photophysics, photochemistry and physical chemistry can be
evaluated in living tissue by fluorescence spectroscopy and imaging
at the intracellular level in terms of structure and function.
Thus, fluorescence adds a new dimension to cell biology and
physiology. This approach is now supported by a full and versatile,
rapidly growing armamentarium of new selective probes for
organelles, enzymes, cations, cytoskeleton and metabolic control.
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Photobiology (Hardcover)
Elli Kohen, Rene Santus, Joseph G. Hirschberg
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R1,274
Discovery Miles 12 740
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Ships in 12 - 17 working days
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Photobiology integrates a wide variety of scientific disciplines.
As more people become aware of the many ways light interacts with
chemical and biological systems, the need for a concise treatment
of photobiology has become more critical. Kohen "et al." Have
written just such a book, intended both as a textbook and as a
reference.
The authors begin by providing a brief description of the nature of
light, how it affects matter, and the means and methods of
measuring it. A major section of the book is devoted to how light
influences living systems, including discussions of photosynthesis,
bioluminescence, regulatory mechanisms, and visual transduction of
light. The last half of the book is devoted to the biomedical
aspects of light, including photoimmunology, photoallergic
reactions and other forms of light sensitivity, the optical
properties of skin, and various ways that light can be used in
therapy treatments.
Useful to photobiologists as a comprehensive overview, this book
should also appeal to biomedical researchers and advanced students
of photobiology.
Containing 100 black and white original photomicrographs accompanied by protocol information, this book shows organelles' structures, interaction, and organization into complexes. It includes a color insert that illustrates some of the most characteristic organelle features, microcompartmentalization, and alterations as investigated by one or more probes simultaneously. Each section provides a brief introduction and technical details of staining methods and gives explanations of unusual appearances and the cytochemical reactions when necessary. The book serves as a guide for therapeutic, diagnostic, or prognostic interpretation of images and for further research.
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