In Gene Therapy Protocols, Volumes 1 & 2, internationally recognized investigators describe cutting-edge laboratory techniques for the study of Production and In Vivo Applications of Gene Transfer Vectors (Volume 1) and Design and Characterization of Gene Transfer Vectors (Volume 2). The field of gene therapy has undergone remarkable advances, promising to impact human healthcare significantly in the twenty-first century. Todaya (TM)s technologies can deliver genetic material safely and effectively to cells to slow or halt the progression of disease, and to help repair or regenerate damaged or lost tissues. In this second volume of Gene Therapy Protocols: Design and Characterization of Gene Transfer Vectors, readers will find a comprehensive resource of current and emerging methods for the processing and characterization of viral and non-viral gene transfer vectors, as well as promising approaches to design vectors for efficient, targeted and regulated gene delivery and expression. This second volume of the new and completely revised third edition of Gene Therapy Protocols will prove a necessary tool for graduate students and postdoctoral fellows and invaluable to basic and clinical researchers in both industry and academia.

In this book internationally recognized investigators describe cutting-edge laboratory techniques for the study of Production and In Vivo Applications of Gene Transfer Vectors and Design and Characterization of Gene Transfer Vectors. Readers will find a comprehensive resource of current and emerging methods for the production of viral and non-viral gene transfer vectors, as well as detailed protocols for applications in stem cell biology, cancer research and infectious disease.

In Gene Therapy Protocols, Volumes 1 and 2, internationally recognized investigators describe cutting-edge laboratory techniques for the study of Production and In Vivo Applications of Gene Transfer Vectors (Volume 1) and Design and Characterization of Gene Transfer Vectors (Volume 2). In this second volume, readers will find a comprehensive resource of current and emerging methods for the processing and characterization of viral and non-viral gene transfer vectors.

Anxiety is the most prevalent psychiatric problem of our time. Decades of research have gone into probing its mysteries and developing treatments. But what if we've been thinking about fear and anxiety in the wrong way for all these years? This is the groundbreaking premise behind a wave of new research, led by the lab of renowned neuroscientist Joseph LeDoux. He believes that fear and anxiety are not innate states, simply waiting to be unleashed in the brain. Rather they are assembled experiences, and that has huge implications for patients. By mapping brain circuits, LeDoux explains the origins of anxiety disorders and reveals discoveries that can restore sufferers to normality. As impressive as it is timely, Anxious is a comprehensive survey of cutting-edge research revolutionising the way we treat our most pressing mental health issue.

"A rigorous, in-depth guide to the history, philosophy, and scientific exploration of this widespread emotional state . . . [LeDoux] offers a magisterial review of the role of mind and brain in the generation of unconscious defense responses and consciously expressed anxiety. . . . [His] charming personal asides give an impression of having a conversation with a world expert." -Nature A comprehensive and accessible exploration of anxiety, from a leading neuroscientist and the author of Synaptic Self Collectively, anxiety disorders are our most prevalent psychiatric problem, affecting about forty million adults in the United States. In Anxious, Joseph LeDoux, whose NYU lab has been at the forefront of research efforts to understand and treat fear and anxiety, explains the range of these disorders, their origins, and discoveries that can restore sufferers to normalcy. LeDoux's groundbreaking premise is that we've been thinking about fear and anxiety in the wrong way. These are not innate states waiting to be unleashed from the brain, but experiences that we assemble cognitively. Treatment of these problems must address both their conscious manifestations and underlying non-conscious processes. While knowledge about how the brain works will help us discover new drugs, LeDoux argues that the greatest breakthroughs may come from using brain research to help reshape psychotherapy. A major work on one of our most pressing mental health issues, Anxious explains the science behind fear and anxiety disorders. Praise for Anxious: "[Anxious] helps to explain and prevent the kinds of debilitating anxieties all of us face in this increasingly stressful world." -Daniel J. Levitin, author of The Organized Mind and This Is Your Brain on Music "A careful tour through the current neuroscience of fear and anxiety . . . [Anxious] will reward the informed reader." -The Wall Street Journal "An extraordinarily ambitious, provocative, challenging, and important book. Drawing on the latest research in neuroscience (including work in his own laboratory), LeDoux provides explanations of the origins, nature, and impact of fear and anxiety disorders." -Psychology Today

The science behind David Goleman's Emotional Intelligence The Emotional Brain provides a cutting-edge scientific background to such books as Daniel Goleman's Emotional Intelligence, showing that while cognitive studies have tended to ignore the emotions, we are increasingly understanding how crucial they are to our evolutionary survival, as shortcuts to cut through conscious reasoning when speed and rules-of-thumb are more important and effective than logic. Much of our emotional life is lived unconsciously, and is far richer than simply our conscious feelings - for example, our conscious mind will already be reacting to situations of danger some time before we begin to be afraid. Not only does LeDoux present a fascinating insight into how our emotions function normally, but also provides a new understanding of emotional disorders.