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This volume focuses on protein analysis, and covers a wide array of
uses of protein microarray for disease analysis. The chapters in
this book discuss different stages of protein microarrays from
their construction to their use, including different types of
protein microarrays such as recombinant proteins, antibody, phage,
and NAPPA protein microarrays, in planar format or in solution via
beads arrays. Written in the highly successful Methods in Molecular
Biology series format, chapters include introductions to their
respective topics, lists of the necessary materials and reagents,
step-by-step, readily reproducible laboratory protocols, and tips
on troubleshooting and avoiding known pitfalls. Comprehensive and
cutting-edge, Protein Microarrays for Disease Analysis: Methods and
Protocols is a valuable resource for graduate and post-doctoral
fellows interested in protein microarrays, as well as senior
researchers interested in gaining more insight into this developing
field.
This volume focuses on protein analysis, and covers a wide array of
uses of protein microarray for disease analysis. The chapters in
this book discuss different stages of protein microarrays from
their construction to their use, including different types of
protein microarrays such as recombinant proteins, antibody, phage,
and NAPPA protein microarrays, in planar format or in solution via
beads arrays. Written in the highly successful Methods in Molecular
Biology series format, chapters include introductions to their
respective topics, lists of the necessary materials and reagents,
step-by-step, readily reproducible laboratory protocols, and tips
on troubleshooting and avoiding known pitfalls. Comprehensive and
cutting-edge, Protein Microarrays for Disease Analysis: Methods and
Protocols is a valuable resource for graduate and post-doctoral
fellows interested in protein microarrays, as well as senior
researchers interested in gaining more insight into this developing
field.
Targeted proteomics is a technology for detecting proteins of
interest with high sensitivity, quantitative accuracy, and
reproducibility. The analysis of a pre-defined group of proteins
provides precise, quantitative, and sensitive data to scientists
and clinicians and can provide information on a subset of proteins
important for their biological function. In this volume, a panel of
high-profile contributors provides an overview of targeted
proteomics in biomedical science. The book is focused on current
state-of-the-art technology and applications, and it will be a
valuable source of information for everyone working in this field.
The first part of the book provides detailed examples of the
application of targeted proteomics in biomarker discovery and
pathogenesis. The second part is focused on the various tools used
in targeted proteomics, including bioinformatics approaches, while
the final section is dedicated to biobanks, a vital aspect of
targeted proteomics. The book is highly recommended for anyone
interested in proteomics, disease pathogenesis, clinical protein
science, and biomarker discovery.
Based on a popular course at Cold Spring Harbor Laboratory, this
new manual assembles cutting-edge protocols, helpful hints, and
lecture notes to teach researchers from a wide variety of
disciplines the essential methods of proteomics using
state-of-the-art instrumentation. Detailed protocols involving
protein microarrays, liquid chromatography, high-throughput cloning
of expression constructs, IMAC, mass spectrometry, MALDI-TOF, and
MudPIT are provided, along with well-illustrated descriptions of
experimental procedures and lists of recommended websites and
reading material. Proteomics: A Cold Spring Harbor Laboratory
Course Manual can be used both as the basis for a course and as a
detailed bench manual for those performing indispensable proteomic
experiments. It is authored by Andrew J. Link, Philip C. Andrews,
and Joshua LaBaer, all leaders in their fields, who bring
complementary expertise to the manual.
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